1644 Results for: "Cell Culture Reagents"

Supplier: Brandtech

Superior meniscus control, and a built-in rest position in a motorized pipette controller.

Supplier: G-Biosciences

Apoptosis is commonly defined as programmed mechanism of cell death and is a highly regulated process that can be initiated by a variety of internal and external stimuli (inducers).

Supplier: G-Biosciences

Apoptosis is commonly defined as programmed mechanism of cell death and is a highly regulated process that can be initiated by a variety of internal and external stimuli (inducers).

Supplier: G-Biosciences

Apoptosis is commonly defined as programmed mechanism of cell death and is a highly regulated process that can be initiated by a variety of internal and external stimuli (inducers).

Supplier: VWR International

These Polypropylene multi-channel reagent reservoirs are designed to support both automated liquid handling platforms and manual systems. The reservoir design complies with ANSI/SLAS 1-2004, when used for cell culture and immunoassay experiments.

Supplier: G-Biosciences

Apoptosis is commonly defined as programmed mechanism of cell death and is a highly regulated process that can be initiated by a variety of internal and external stimuli (inducers).

Supplier: Promega Corporation

The MultiTox-Fluor Multiplex Cytotoxicity Assay is a single-reagent-addition, homogeneous, fluorescent assay that measures the number of live and dead cells simultaneously in culture wells.

Supplier: G-Biosciences

Apoptosis is commonly defined as programmed mechanism of cell death and is a highly regulated process that can be initiated by a variety of internal and external stimuli (inducers).

Supplier: G-Biosciences

Apoptosis is commonly defined as programmed mechanism of cell death and is a highly regulated process that can be initiated by a variety of internal and external stimuli (inducers).

Supplier: Promega Corporation

Promotes rapid lysis of cultured mammalian cells without the need to scrape adherent cells or freeze-thaw. Lytic reagent of choice when processing cells for quantitation of firefly and Renilla luciferase activities using the Promega Dual-Luciferase® Reporter Assay (#PAE1910).

Supplier: OZ Biosciences

3D-Fect is specifically developed to directly transfect cells cultured in 3D scaffolds.

Supplier: Bio-Rad

iScript™ RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis, RNA stabilization, and removal of genomic DNA for sensitive qPCR without traditional RNA sample preparation.

Supplier: Cell Biolabs

Cell Biolabs’ CytoSelect™ MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1).  An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells

Supplier: Abcam

MTS reagent for cell proliferation assay. Tetrazolium inner salt is used for determining cell viability in proliferation assays. Reduced in metabolically active cells to a colored formazan product that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes. - Purity >98% - Soluble in DPBS to 2 mg/ml

Supplier: MP Biomedicals

Aprotinin is a single chain polypeptide (58 amino-acids) crosslinked by three disulfide bridges, showing competitive and reversible inhibiton of proteolytic and esterolytic activity. Aprotinin forms stable complexes with, and blocks the active sites of serine protease enzymes. It is found in bovine lymph nodes, lung, parotid gland, spleen, liver, pancreas, seminal vesicles, thyroid gland, kidney, mucous membranes of the trachea and esophagus, ovaries, heart, posterior pituitary and cartilage.

Supplier: Cayman Chemical Company

Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

Supplier: BIOASSAY SYSTEMS

Quantitative determination of L-amino acids in biological and other samples.

Supplier: Invitrogen

The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.

Supplier: Spectrum Chemical Mfg. Corp.

Sodium Pyruvate, Reagent is most often added to the cell culture media as an extra source of energy. Sodium pyruvate also has a protective action against hydrogen peroxide. The Reagent grade denotes that this chemical is the highest quality commercially available and that the American Chemical Society has not officially set any specifications for this material. Spectrum Chemical manufactured Reagent grade products meet the toughest regulatory standards for quality and purity.

Supplier: Corning

Cellstripper® is a non-enzymatic cell dissociation solution formulated with a proprietary mixture of chelators that gently dislodge adherent cells in tissue culture. It is an alternative to trypsin when used with either serum-free or serum-supplemented media. Because Cellstripper® is a gentler reagent than trypsin, cells can be exposed to Cellstripper for longer periods of time. Cellstripper® achieves superior results in comparison to enzymatic dissociation products.

Supplier: Invitrogen

The Mem-PER™ Plus Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. It effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure.

Supplier: Adipogen

Reagent for the spectrophotometric det. of Ag and Ru. Tridentate ligand that binds metals at three meridional sites giving two adjacent 5-membered MN2C2 chelate rings. It forms complexes with most transition metal ions. It is also a potent inducer of nitrate and nitric oxide (NO) synthase (iNOS) protein and mRNA production in the cultured murine macrophage RAW 264.7 cell line.

Supplier: Beckman Coulter

VersaLyse Lysing solution is a reagent used to lyse red blood cells from any biological fluid and, in particular, to lyse erythrocytes from whole blood. VersaLyse is a highly specific, very gentle lysing solution, suitable for applications such as immunophenotyping, cell sorting, activation or cell culture. It is designed for use in flow cytometry and facilitates obtaining highly discriminative scattergrams whatever the type of flow cytometer used. VersaLyse is a versatile reagent which may be used in lyse/wash, wash/lyse and lyse/no-wash procedures with undiluted or diluted samples. The histograms are dual-parameter light scatter histograms (FS versus SS) of a normal K3EDTA anticoagulated blood sample lysed with VersaLyse Lysing Solution (No-wash procedure).

Supplier: ARBOR ASSAYS MS

The Formaldehyde Fluorescent Detection Kit from Arbor Assays provides a sensitive, high-throughput method to detect formaldehyde in urine and tissue culture media (TCM). Using a proprietary fluorescent reagent, the assay quantifies formaldehyde levels down to 0.715 µM with a broad detection range of 3.125 to 200 µM. With a simple 30-minute workflow and low background fluorescence, the assay is ideal for studying metabolic processes, toxicology, and cell culture contamination. The kit uses a kinetic fluorescent readout, providing reliable and reproducible results suitable for both research and screening applications.

Supplier: Sartorius

The Vivapure® AdenoPACK™ adenovirus purification and concentration kits provides researchers who need to recover up to 3×10¹³ purified recombinant adenovirus particles for in vitro transfection a fast, safe and easy to use solution. The kits include all reagents and devices necessary for clarification, purification and concentration of adenovirus type 5 from HEK293 cell cultures.

Supplier: Harvard Apparatus

BTXpress Cytoporation® Media T/T4 is an advanced electroporation buffer designed for use with the BTX AgilePulse MAX™ large volume electroporation system for in vitro delivery of DNA, RNA, oligonucleotides, and siRNA. The low conductivity buffer is specially formulated to minimise heating of solution during large volume electroporation for maximum transfection efficiency and high cell viability. BTXpress Cytoporation® Media T and T4 are sterile filtered from the highest quality non animal, medical grade reagents. Buffer can be directly diluted in complete growth media for post electroporation cell culture.

Supplier: RPI

PBS 10X solution, is a widely used reagent in life science research. It is a buffered saline solution that contains phosphate ions, which help maintain a stable pH level in biological and biochemical experiments. PBS 10X solution is commonly used in various applications such as cell culture, immunohistochemistry, immunocytochemistry, and molecular biology techniques. The primary function of PBS 10X solution is to provide a stable environment for biological samples by maintaining a physiological pH and ionic strength.

Supplier: Advanced Biomatrix

PureCol® EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator. The product consists of purified Type I collagen at a concentration of 0.5%, (~5 mg/ml), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture.
PureCol® EZ Gel is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions.

PureCol® EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. PureCol® EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.

Supplier: Peprotech

TWEAK belongs to the TNF family of ligands, and signals through TWEAKR, also known as TNFRSF12A. TWEAK is expressed in a variety of tissues, including the adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK has the ability to induce NF-κB activation and chemokine secretion, and to exert an apoptotic activity in certain cells, such as HT-29 human adenocarcinoma cells when cultured in the presence of IFN-γ. TWEAK also promotes proliferation and migration of endothelial cells. The human TWEAK gene encodes for a 249 amino acid type II transmembrane protein, which contains a 21 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 207 amino acid extracellular domain. Recombinant Human TWEAK is a soluble 17.0 kDa polypeptide (154 amino acid residues) comprising the TNF-homologous region of TWEAK, and is generated by proteolytic processing of the full length membrane-anchored TWEAK protein.Manufactured using all Animal-Free reagents.

Supplier: Adipogen

This common fluorescent reagent often used in carbohydrate analysis, is also useful in fluorescence visualization of lysosomes in live cell lines in culture. This fluorophore that has the unusual environmentally sensitive property of increasing fluorescence in low pH environments. Comparison of staining patterns with the common LysoTracker™ or LysoSensor™ dyes indicated comparable staining, albeit using a higher concentration of NBD-piperazine (1μM versus 100nM for the Lyso-Dyes) but at a lower cost per assay. Also used for the determination of mono- and diisocyantes in air samples, and determination of lactate, hydroxybutyrate and other intermediates in body fluids. The piperazino moiety reacts readily with isocyantes to form the corresponding urea-derivatives. The derivatives can be detected by LC with UV or fluorescent-detection. Spectral data: lambdaex 470nm, lambdaem 540nm.