1638 Results for: "Cell Culture Reagents"

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C)
L-Glutamine, the uncharged and amidated analog of L-glutamic acid, is an important amino acid for the incorporation of NH4+ into biomolecules. It is biosynthesized from NH4.
L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture.
L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Supplier: MP Biomedicals

Storage: Store at room Temperature (15-30 °C)
Sucrose is a nonreducing disaccharide composed of glucose and fructose, linked via their anomeric carbons. It is obtained commercially from Sugarcane, sugar beet (Beta vulgaris), and other plants and used extensively as a food and a sweetener.
Use to create sucrose gradients for purification of viruses and proteins.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C).
Methionine is one of the common sulfur-containing amino acids. Methionine is a common methyl-group donor to various substrates, such as creatine, epinephrine, ergosterol, and choline.

Supplier: MP Biomedicals

Storage: +4 °C
D-Biotin is a growth factor present in small amounts in every living cell. It is involved in naturally occurring carboxylation reactions. It occurs mainly bound to proteins or polypeptides. It is more abundant in the liver, kidney, pancreas, yeast and milk. Biotin levels are higher in cancerous tumors than in normal tissues. It is inactivated by binding to avidin.
D-Biotin may be used to elute proteins from avidin/streptavidin resins. It is widely used for dietary supplements and fortified foods. It is also used for tablets and hard-shell capsule preparation due to its pharmaceutical properties.
Essential vitamin that is important for amino acid and energy metabolism, and fatty acid synthesis. It is a prosthetic group in four mammalian carboxylase families and facilitates the binding and transfer of carbon dioxide.
Soluble in water (22 mg/100 mL), ethanol (80 mg/100 mL), more soluble in hot water and in dilute alkalies; insoluble in other common organic solvents. Soluble in 2 M Ammonium hydroxide (50 mg/mL - clear, colorless solution), dimethylformamide (1.7 mg/mL).

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C)
Sodium chloride is a commonly used chemical which is found widely in nature. It is considered to be an essential nutrient. Excess amounts of sodium chloride can destroy electrolyte balance and cause death in most animals, including humans.
Sodium chloride is used in a wide variety of biochemical applications, including intravenous fluids (normal saline is 0.9% w/v in water), density gradients, a diluent to increase ionic strength in buffers or culture media and in salt-out procedures in the isolation of DNA. It has also been used in high concentrations for preservation of foods since bacteria cannot grow in high salt conditions. Sodium chloride is widely used in protein crystallization.

Supplier: MP Biomedicals

Storage: -20°C, desiccate
This is an ultrapure NAD, chromatographically purified to remove trace inhibitors.
β-NAD, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. It occurs in living cells primarily in the oxidized state. Serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols. NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain.
Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.
Electron acceptor. β-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.

Supplier: MP Biomedicals

L-Glutamic Acid is a non-essential amino acid for human development; referred to as an excitatory amino acid (EAA) due to its role in neurotransmission. Acts as a neurotransmitter at fast synapses; agonist at kainate, NMDA and quisqualate receptors. L-Glutamic acid is one of the two amino acids that contains a carboxylic acid group in its side chains.

Supplier: QUALITY BIOLOGICAL, INC.

QBI’s Water, Cell Culture, WFI Quality is high quality water for use as a solvent in the preparation of cell culture media and biochemical reagents.

This water is extensively purified by reverse osmosis, subsequently deionized and passed through a filter membrane to remove particles and tested to meet the specifications found in the USP (United States Pharmacopeia) monograph for Water for Injection (WFI) for conductivity, endotoxin,and TOC. It is additionally tested for sterility, mycoplasma, and cell culture performance.

This water is tested against USP specifications, but is not intended for injection. It is only intended for research or further manufacturing use, not for diagnostic or therapeutic use.

Supplier: Thermo Scientific Chemicals

Macrolide antibiotic that inhibits bacterial protein synthesis.

Supplier: MP Biomedicals

Dimethyl Sulfoxide is ideal for use as a cryoprotectant. Also used in chemical reactions, in polymerase chain reactions (PCR) as a PCR cosolvent to help improve yields, especially in long PCR, and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal-induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines, embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS). Used to enhance dermal absorption of many chemicals. A solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers. Used in antifreeze or hydraulic fluids. A cryopreservative for cell cultures. Used in the oxidation of thiols and disulfides to sulfonic acids.

Dimethyl sulfoxide (DMSO) is a highly polar aprotic organic reagent that has exceptional solvent properties for organic and inorganic chemicals

Physical Apppearance: Liquid
Viscosity: 1.1 centipoises (27 °C)
Vapor Density: 2.7 (vs air)
Vapor Pressure: 0.55 hPa at 20 °C, 0.42 mmHg (20 °C)
Refractive Index: n20/D 1.479(lit.)
Solubility: Soluble in water (1 mL DMSO + 1 mL H2O), methanol, acetone, ether, benzene, chloroform

Supplier: Thermo Scientific Chemicals

Powder

Supplier: Thermo Scientific Chemicals

Non-animal sourced L-cystine is used as a cell culture media component for the commercial biomanufacture of therapeutic recombinant proteins and monoclonal antibodies. L-cystine is used as a cell culture supplement.

Supplier: Thermo Scientific Chemicals

A lipotropic agent

Supplier: Thermo Scientific Chemicals

Powder

Supplier: Corning

Cell culture grade water is high-quality water suitable for use as a solvent in the preparation of cell culture media and laboratory reagents.

Supplier: Thermo Scientific Chemicals

An aminocyclitol antibiotic that binds to the 30S ribosomal subunit of bacteria and interrupts protein synthesis

Supplier: MP Biomedicals

D-(+)-GALACTOSE, Purity: >/= 98%, CAS Number: 59-23-4, Molecular Formula: C6H12O6, Molecular Weight: 180.2, Grade: Anhydrous, Cell culture reagent, Appearance/Format: White powder, Storage: Room Temperature (15-30 deg C), Size: 500 g

Supplier: MP Biomedicals

Cytidine is a pyrimidine nucleoside that is composed of the base cytosine linked to the five-carbon sugar d-ribose. There are a variety of cytidine analogs with potentially useful pharmacology .
Cytidine when intraperitoneal administered in combination with choline improves nerve regeneration and functional recovery in a rat model of sciatic nerve injury. Used as an ex vivo drug selectable marker in gene-modified primary bone marrow stromal cells for cytidine deaminase assay.
Physical Appearance: White Powder
Optical Rotation: +34.6 ° (Lit.)
UV/Visible Absorbance: λ max (water): 270 - 272 nm
Solubility: Soluble in water (50 mg/mL- clear colorless to faint yellow solution)

Supplier: Corning

L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing toxic compounds. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Supplier: Corning

100 mM Solution with 8.5 g/L NaCl Sodium pyruvate is often used as a component in culture broth and media as an energy source for propagating cells. This reagent is also used in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid.

Supplier: Corning

Cell culture grade water is high-quality water suitable for use as a solvent in the preparation of cell culture media and other reagents for sensitive applications. Our bioprocess container selections are suitable for large-scale applications such as cleaning and upstream and downstream reagent preparation. Cell culture grade water goes through a wide array of testing which includes testing to the chemical and physical properties found in the USP and EP monographs for sterile Water for Injection (WFI).

Supplier: IBI Scientific

IBI gPURE DNA Isolation Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial, or viral DNA

Supplier: Abcam

3D Cell Culture Clearing Reagent ab243302 is part of our range of reagents that enable you to set up tissue clearing, and to clear 3D cell cultures, easily and quickly.

Supplier: MP Biomedicals

Calcium Chloride, Dihydrate, Purity: approx 99%, Cell Culture Reagent, CAS Number: 10035-04-8, Molecular Formula: CaCl2.2H2O, Molecular Weight: 147, Synonyms: Calcium dichloride dihydrate, Color: White, Form: Powder, size: 5 kg

Supplier: AAT Bioquest

Hank's Balanced Salt Solution with 20 mM Hepes buffer (HHBS) is used for a variety of cell culture applications, such as washing cells, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

Supplier: MP Biomedicals

Uridine is a nucleoside, contains an uracil attached to a ribose ring (known as a ribofuranose) via a β-N1-glycosidic bond. It is an organic compound of the pyrimidine family, that occurs as one of the four bases coding genetic information in the polynucleotide chain of RNA.
Uridine can increase the rate-limiting step of Phosphatidylcholine synthesis in the brain, Phosphatidylcholine is the major structural and functional component of brain cell membranes. Without phosphatidylcholine, brain cells lose their youthful function and suffer the degenerative structural changes associated with aging. Uridine is thought to aid Alzheimer's Disease due to helping with synaptic connections, which appear to be reduced in the state of Alzheimer's.
Physical Appearance: White Powder
UV/Visible Absorbance: λ max (water)= 260 - 263 nm
Solubility: Soluble in 0.1 N HCl (50 mg/mL-clear, colorless solution) and water (50 mg/mL)

Supplier: Cell Systems

Optimize the plating, passaging and freezing of cell cultures with Cell Systems Reagents. Growth supplements and antibiotic products are also available to ensure each experiment is initiated in an optimal environment.

Supplier: MP Biomedicals

Cycloheximide is a glutarimide antibiotic derived from a microbial source. Cycloheximide is an antibiotic which is very active against many molds, yeasts, and phytopathogenic fungi. It exhibits somewhat lower activity against bacteria and certain fungi. Control of various molds and fungi in gelatin-based photographic emulsions, photoengraving glues, and other light-sensitive products is suggested.
Cycloheximide is used in plant research to study disease resistance and as an ethylene stimulant, useful in studies involving fruit and leaf production. It is also used in bacteriological media to isolate or count bacteria in the presence of yeast and molds; Used in protein synthesis in apoptosis; Gene expression; Glycogenolysis, gluconeogenesis and ureogenesis in isolated rat hepatocytes; Studies involving steroidogenesis; Used in plant regulation and as a quality control measure by the food and beverage industry.
Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors. Cycloheximide inhibits peptide synthesis in eukaryotic organisms but not in prokaryotes. Protein synthesis is blocked by the interaction of cycloheximide with the translocase enzyme. This interaction prohibits the translocation of messenger RNA on the cytosolic, 80S ribosomes without inhibiting organelle protein synthesis. Cycloheximide is also known to induce FAS/FAS Ligand apoptosis, and triggers apoptosis in HL-60 cells, T-cell hybridomas, Burkitt's lymphoma cells in addition to a variety of other cell types. Cycloheximide will also delay or inhibit apoptosis induced by other agents.

Supplier: MP Biomedicals

Storage: Room Temperature
2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its diminished vapor pressure, its odour, while unpleasant, is less objectionable than related thiols.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also it reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Supplier: MP Biomedicals

Storage: Store at room temperature (15-30 °C)
L-Lysine monohydrochloride is widely used as nutritional supplements in food and beverage industries. It can also be used in animal feed as source of L-Lysine. L-Lysine Monohydrochloride can be used in a wide variety of industries including: food production, beverage, pharmaceutical, agriculture/animal feed, and various other industries.
L-Lysine monohydrochloride is a key amino acid in calcium absorption.