127859 Results for: "Blotting"

Supplier: Abcam

Avidin/biotin blocking kit blocks signals from endogenous avidin, biotin and biotin-binding proteins in tissues when used with biotin-based IHC detection. - Cited in over 35 publications - Ideal for tissues rich in biotin (e.g., liver, kidney) - Easy-to-use, quick protocol

Supplier: Enzo Life Sciences

Species Independent

Supplier: Enzo Life Sciences

Whole-cell extract of human HL-60 leukemia cells.

Supplier: AAT Bioquest

Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.

Supplier: ADVANSTA INC MS

Sirius is the most sensitive chemiluminescent substrate from Advansta.

Supplier: ADVANSTA INC MS

Kit for fast, easy concentration and purification of samples for SDS-PAGE.

Supplier: MilliporeSigma

Fluorogenic caspase-1 substrate

Supplier: Abcam

This product is a liquid substrate for alkaline phosphatase (AP), and is especially useful for the detection of AP-conjugated antibodies.

Supplier: MilliporeSigma

Fluorogenic proteasome substrate.

Supplier: Invitrogen

The background eliminator reduces background signal evenly over any film within minutes

Supplier: Invitrogen

LightShift poly(dI-dC) can be used as competitor for nonspecific DNA binding proteins.

Supplier: Surmodics

DAB membrane substrate is supplied as a two component liquid substrate system designed for the rapid detection of peroxidase in membrane applications.

Supplier: MilliporeSigma

A fluorogenic substrate for the detection of granzyme B and caspase-8 activity

Supplier: Abcam

The Optiblot SDS-PAGE Sample Preparation Kit (ab133414) provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis.

Supplier: MilliporeSigma

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6)

Supplier: MilliporeSigma

A fluorogenic substrate for detecting caspase-1 (ICE), caspase-4, and caspase-5 activity

Supplier: Alkali Scientific

Optimize your chemiluminescent assays with the Femto HRP Chemiluminescent Substrate Kits, providing sensitive detection for various applications.

Supplier: MilliporeSigma

Colorimetric substrate for the detection of granzyme B and caspase-8 activity. Cleavage of pNA is monitored colorimetrically at ~405nm.

Supplier: Enzo Life Sciences

Used in conjunction with peroxidase-based immunostaining systems.

Supplier: Invitrogen

The substrate generates a blue fluorescent product upon reaction with peroxidase and does not photobleach.

Supplier: Invitrogen

Thermo Scientific Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

Supplier: Invitrogen

Thermo Scientific Pierce Firefly Signal Enhancer is designed to complement the Pierce Firefly Luciferase Flash and Glow Assay and is recommended when maximum sensitivity is desired.

Supplier: Cytiva

Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.

Supplier: G-Biosciences

Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis

Supplier: Abcam

Antibody diluent (pH 7 - 7.8) is used to dilute primary antibodies to their working concentrations for use in immunohistochemistry (IHC). - Cited in over 15 publications - Reduces background and non-specific binding - Extends antibody stability up to 24 months at 2-8°C - Tris-buffered with stabilizing protein, wetting agent, antimicrobial agent, and green dye

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Supplier: Rockland Immunochemical

Enzyme Substrates, Rockland Immunochemical

Supplier: Thermo Scientific Chemicals

Saponin permeating solution, is used as a cell permeabilizing agent.

Supplier: MilliporeSigma

A fluorogenic substrate for caspase-6 (Mch2)