127859 Results for: "Blotting"

Supplier: Abcam

NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting.

Supplier: Abcam

Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is a sensitive immunoenzymatic system for detecting antibodies bound to antigens within tissue sections by light microscopy.

Supplier: Invitrogen

Thermo Scientific Guardian Peroxidase Conjugate Stabilizer/Diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long-term storage.

Supplier: Advansta

Increased protein retention on the membrane and fast transfer times result in more sensitive detection of low-abundance and post-translationally modified proteins

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Invitrogen

The Thermo Scientific LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

Supplier: G-Biosciences

G-Biosciences' Western ReProbe™ is a single component system, specifically formulated to dissociate and remove antibodies from membrane bound proteins without destroying the antigenic binding affinity and does not use denaturants, SDS or boiling

Supplier: Vector Laboratories

Vector Laboratories alkaline phosphatase (AP) substrate options include standard substrates and ImmPACT substrates with higher sensitivity. The range of vibrant colors facilitates multiplex staining and improved contrast with pigmented tissues and counterstains.

Supplier: Invitrogen

NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt

Supplier: Bio-Rad

Bio-Rad Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP) Secondary Antibody Conjugates are engineered for use with blot detection protocols including colorimetric and chemiluminescent.

Supplier: Invitrogen

Thermo Scientific HisProbe-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.

Supplier: Advansta

WesternBright ECL is a horseradish peroxidase substrate optimized for chemiluminescent Western blots imaged using x-ray film. WesternBright ECL requires as little as ten times less antibody than other substrates, allowing you to save precious antibodies and samples. Additionally, the WesternBright ECL signal is long lasting, allowing multiple exposures to be carried out without substantial signal decay.

Supplier: Abcam

AEC substrate kit for use in IHC using HRP / peroxidase detection.

Supplier: MilliporeSigma

Fluorogenic caspase-3 substrate

Supplier: Alkali Scientific

Achieve sensitive detection in chemiluminescence assays with the BrightStar™ Duration HRP Chemiluminescent Substrate, designed for accurate and efficient protein detection.

Supplier: Invitrogen

The Thermo Scientific SuperSignal West HisProbe Kit contains HisProbe-HRP Conjugate, as well as reagents required for detection of polyhistidine-tagged protein by Western blotting

Supplier: Cytiva

CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.

Supplier: Surmodics

Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in ELISA and membrane assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the dioxetane substrate, antibody/antigen titers and blockers. Surmodics provides multiple 1-component AP chemiluminescent substrates with various intensities and wavelength emissions to allow the assay developer to choose the appropropriate substrate for the application.

Supplier: Abcam

Ultra High Sensitivity ECL Substrate Kit ab133409 is a horseradish peroxidase (HRP) substrate specially developed for Western blotting detection of very low abundance proteins with 1.

Supplier: Surmodics

Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in ELISA and Western Blot assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the luminol substrate, antibody/antigen titers and blockers. Surmodics provides multiple 2-component HRP chemiluminescent substrates with various intensities to allow the assay developer to choose the appropropriate substrate for the application.

Supplier: Electron Microscopy Sciences

Improving Agarose Gel Performance

Supplier: Abcam

High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band.

Supplier: MilliporeSigma

Fluorogenic caspase-3 substrate

Supplier: Invitrogen

Thermo Scientific SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) HRP substrate for picogram to high-femtogram level detection.

Supplier: Invitrogen

QuantaBlu™ NS/K fluorogenic substrate kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays

Supplier: MilliporeSigma

Genomic DNAs are highly purified preparations of high molecular weight genomic DNA from a variety of organisms.

Supplier: G-Biosciences

Perfect-FOCUS™ is designed to clean and concentrate protein samples that give poor protein spot resolution during 2D electrophoresis

Supplier: KPL

SureBlue Reserve™ provides maximum sensitivity for detection of peroxidase labeled conjugates in a microwell format and provides 50% more sensitivity than standard SureBlue™ TMB substrate

Supplier: Abcam

Avidin/biotin blocking kit blocks signals from endogenous avidin, biotin and biotin-binding proteins in tissues when used with biotin-based IHC detection. - Cited in over 35 publications - Ideal for tissues rich in biotin (e.g., liver, kidney) - Easy-to-use, quick protocol

Supplier: Invitrogen

The Thermo Scientific Pierce DAB Substrate Kit enables chromogenic detection of horseradish peroxidase (HRP) activity based the action of 3,3'-diaminobenzidine (DAB) in Western blot and tissue staining methods.