108 Results for: "Bioconjugation Reagents"
5-Carboxyrhodamine 110 (5-CR110) single isomer ≥95%
Supplier: AAT Bioquest
Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength.
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6-Carboxyrhodamine 110 (6-CR110) single isomer ≥95%
Supplier: AAT Bioquest
Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength.
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5(6)-TAMRA mixture of isomers ≥85%
Supplier: AAT Bioquest
Tetramethylrhodamine-5-(and-6)-maleimide is an excellent reagent for preparing orange fluorescent bioconjugates through thiol modifications of peptides, nucleotides, nucleic acids and proteins.
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6-Carboxy-4,7-dichlororhodamine 110 (6-dR110) ≥95%
Supplier: AAT Bioquest
Compared to fluorescein labeling reagents such as FITC and FAM, 6-dR110 [6-carboxy-4,7-dichlororhodamine 110] reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength.
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5-Carboxy-4,7-dichlororhodamine 110 (5-dR110) ≥95%
Supplier: AAT Bioquest
Compared to fluorescein labeling reagents such as FITC and FAM, 5-dR110 [5-carboxy-4,7-dichlororhodamine 110] reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength.
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5-dR110 succinimidyl ester ≥75%
Supplier: AAT Bioquest
Compared to fluorescein labeling reagents such as FITC and FAM, 5-dR110 [5-carboxy-4,7-dichlororhodamine 110] reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength.
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5(6)-Tetramethylrhodamine isothiocyanate [5(6)-TRITC] ≥90%
Supplier: AAT Bioquest
5(6)-TRITC is an amino-reactive labeling reagent that is widely used in preparing bioconjugates of proteins and nucleic acids.
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5(6)-Carboxytetramethylrhodamine succinimidyl ester [5(6)-TAMRA SE] ≥95%
Supplier: AAT Bioquest
The succinimidyl esters of 5-TAMRA, 6-TAMRA or the mixed isomers are the primary labeling reagents for the preparation of orange fluorescent bioconjugates, including peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry.
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Tetramethylrhodamine 6-isothiocyanate chloride ≥90%
Supplier: AAT Bioquest
6-TRITC (also called R isomer) is the other isomer of the TRITC labeling reagent that is widely used in preparing bioconjugates of proteins and nucleic acids.
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Sulfo-NHS (N-Hydroxysulfosuccinimide sodium salt), Pierce™
Supplier: Invitrogen
Pierce™ Sulfo-NHS (N-hydroxysulfosuccinimide) is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.
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5(6)-Carboxyfluorescein ≥95%
Supplier: AAT Bioquest
Carboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations.
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5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5/6-FAM SE) ≥95%
Supplier: AAT Bioquest
Carboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations.
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5(6)-ROX N-succinimidyl ester mixture of isomers ≥90%
Supplier: AAT Bioquest
5(6)-ROX, SE is the succinimidyl esters of 5(6)-ROX.
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Ethylene glycol diglycidyl ether (so called)
Supplier: TCI America
CAS Number: 2224-15-9 MDL Number: MFCD00005142 Molecular Formula: C8H14O4 Form: Clear Liquid Color: Colorless Flash Point (°C): 157 Specific Gravity (20/20): 1.19
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5(6)-Carboxyrhodamine 110, HCl salt
Supplier: Anaspec
Although FITC reagents have been predominantly used to prepare green fluorescent bioconjugates, the low photostability and pH-dependent fluorescence of FITC bioconjugates make researchers search for alternative fluorophores in bioconjugations. Among green fluorophores, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength.
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Pierce™ NHS-Azide and NHS-Phosphine Reagents, Thermo Scientific
Supplier: Invitrogen
The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.
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5(6)-Carboxyfluorescein
Supplier: Anaspec
Although FITC reagents have been more often used to prepare fluoresceinated bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions and give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.
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5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5/6-FAM SE)
Supplier: Anaspec
Although FITC reagents have been more often used to prepare fluoresceinated bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions and give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.
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N-Hydroxysuccinimide, Pierce™
Supplier: Invitrogen
Thermo Scientific Pierce NHS is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.
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Fluorescein-5-maleimide ≥90% (by HPLC)
Supplier: Adipogen
Green fluorescent thiol-reactive dye (Ex/Em: 494/519nm) for bioconjugation, widely used as protein and peptide fluorescent labeling reagent, easily conjugating fluorescein. The reaction requires very mild condition and is highly specific.
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DACM ≥98% (by NMR)
Supplier: Adipogen
Excellent blue fluorescent thiol-reactive reagent (Ex/Em: 383/463nm), widely used for probing configurations of biomolecules such as proteins and create blue-fluorescent bioconjugates. The unreacted reagent is non-fluorescent and can also be used to quantitate free thiols. Shows superior fluorescence and water solubility properties than dansyl chloride.
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3-Carboxy-6,8-difluoro-7-hydroxycoumarin ≥98% (by HPLC)
Supplier: Adipogen
This amine-reactive reagent can be used to create blue-fluorescent bioconjugates with excitation/emission maxima ~410/455 nm that are excitable by the 405 nm spectral line of the blue diode (violet) laser. Used in highly sensitive analyses of amines, amino acids and dipeptides. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the amine terminus of proteins and the є-amino group of lysines.
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Fmoc-Ala-Ala-OH
Supplier: Chem-Impex International
Fmoc-Ala-Ala-OH is a highly regarded compound in the field of peptide synthesis, known for its role as a versatile building block in the development of peptides and proteins. This compound features a fluorenylmethoxycarbonyl (Fmoc) protecting group, which is essential for the selective protection of amino groups during the synthesis process. Its unique structure allows for efficient coupling reactions, making it an ideal choice for researchers and professionals engaged in peptide chemistry. Fmoc-Ala-Ala-OH is particularly useful in the synthesis of dipeptides and can be employed in various applications, including drug development, bioconjugation, and the design of peptide-based therapeutics. Its stability and compatibility with standard coupling reagents enhance its utility in complex peptide assembly, providing a reliable option for achieving high yields in synthetic processes. Moreover, Fmoc-Ala-Ala-OH stands out due to its ease of use and the ability to facilitate the synthesis of peptides with specific sequences, which is crucial for the development of targeted therapies. Researchers appreciate its effectiveness in generating peptides that can be utilized in a range of applications, from basic research to advanced therapeutic solutions. With its proven track record in peptide synthesis, Fmoc-Ala-Ala-OH is an indispensable tool for professionals looking to innovate in the fields of biochemistry and pharmacology.
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Chemical Phosphorylation Reagent I (CPR I)
Supplier: AAT Bioquest
This reagent can be used to create the 3'-phosphate by adding it as the first addition to the support.
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5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB) ≥98.0% (by HPLC, titration analysis)
Supplier: TCI America
[for Determination of SH groups] CAS Number: 69-78-3 MDL Number: MFCD00007140 Molecular Formula: C14H8N2O8S2 Molecular Weight: 396.34 Purity/Analysis Method: 98.0% (HPLC,T) Form: Crystal
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NAM [=N-(9-Acridinyl)maleimide] ≥98.0% (by HPLC)
Supplier: TCI America
[for HPLC Labeling] CAS Number: 49759-20-8 MDL Number: MFCD00042983 Molecular Formula: C17H10N2O2 Molecular Weight: 274.28 Purity/Analysis Method: 98.0% (HPLC) Form: Crystal
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N-Succinimidyl S-Acetylthioglycolate (SATA) ≥94.0%
Supplier: TCI America
[Cross-linking Reagent] CAS Number: 76931-93-6 MDL Number: MFCD00036891 Molecular Formula: C8H9NO5S Molecular Weight: 231.22 Purity/Analysis Method: 94.0% (GC) Form: Crystal Color: White Melting point (°C): 97 Storage Temperature: 0°C
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PE, ReadiUse™ fluorescent probe, preactivated
Supplier: AAT Bioquest
R-Phycoerythrin (PE) is isolated from red algae.
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DSP (Dithiobis(succinimidyl propionate)) ≥97.0% (by total nitrogen basis)
Supplier: TCI America
[Cross-linking Reagent] CAS Number: 57757-57-0 MDL Number: MFCD00042045 Molecular Formula: C14H16N2O8S2 Molecular Weight: 404.41 Purity/Analysis Method: 97.0% (N) Form: Crystal Melting point (°C): 133 Storage Temperature: 0-10°C
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Pierce™ PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, Thermo Scientific
Supplier: Invitrogen
Pierce™ SAT(PEG)₄ (PEGylated N-succinimidyl S-acetylthioacetate) is a sulphhydryl-addition reagent for covalent modification of primary amines.