1012 Results for: "Biochemical Reagents"

Supplier: Hardy Diagnostics

P-dimethylaminobenzaldehyde, to test indole production in indole broth or for biochemical strip/cupule methods

Supplier: Indofine Chemical Company

Rare Organics & BioChemicals 1gm MBTH Room temperature.

Supplier: TCI America

[for Biochemical Research]
CAS Number: 298-83-9
MDL Number: MFCD00012159
Molecular Formula: C40H30Cl2N10O6
Purity/Analysis Method: >98.0% (HPLC,T)
Form: Crystal
Melting point (°C): 223
Storage Temperature: <0°C

Supplier: Thermo Scientific Chemicals

Dithiothreitol (DTT, Cleland's reagent) 99% for biochemistry

Supplier: WORTHINGTON BIOCHEMICAL CORP MS

Papain dissociation system is a set of reagents intended for use in the neural cell isolation method of Huettner and Baughman. Papain is used with fetal as well as postnatal brain regions to provide maximal dissociation and viability of neurons.

Supplier: MP Biomedicals

Proteinase K is a highly active stable endopeptidase with a broad spectrum of action was isolated by E. Merk's Darmstadt Biochemical Research Department in 1970 from a culture filtrate of the fungus, Tritirachium album Limber. This fungus is able to grow on Keratin (e.g., wool, horn particles) as the sole source of carbon and nitrogen. The isolated protease was, therefore, given the K designation.

Supplier: Spectrum Chemical Mfg. Corp.

Dextrose, Anhydrous, Granular, BiotechGrade is a simple monosaccharide sugar and is commonly referred to as glucose. The critical parameters involved are absence of inhibitors such as traces of heavy metals as well as biochemical function tests for enzymes, coenzymes and enzyme substrates.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C)
Sodium chloride is a commonly used chemical which is found widely in nature. It is considered to be an essential nutrient. Excess amounts of sodium chloride can destroy electrolyte balance and cause death in most animals, including humans.
Sodium chloride is used in a wide variety of biochemical applications, including intravenous fluids (normal saline is 0.9% w/v in water), density gradients, a diluent to increase ionic strength in buffers or culture media and in salt-out procedures in the isolation of DNA. It has also been used in high concentrations for preservation of foods since bacteria cannot grow in high salt conditions. Sodium chloride is widely used in protein crystallization.

Supplier: Spectrum Chemical Mfg. Corp.

Sodium Bicarbonate, Powder, BiotechGrade is a commonly used laboratory chemical that can react with both acids and bases, therefore it can be used as a neutralizer. The critical parameters involved are absence of inhibitors such as traces of heavy metals as well as biochemical function tests for enzymes, coenzymes and enzyme substrates.

Supplier: G-Biosciences

Lysis and extraction of biologically active proteins from cellular and tissue samples is the first critical step for biochemical analysis

Supplier: Electron Microscopy Sciences

Luft's Formula.

Supplier: REVVITY HEALTH SCIENCES, INC.

The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. The VICTOR Nivo is a high-performance multimode microplate reader that can be equipped with all major detection technologies: Absorbance, Luminescence, Fluorescence, Time-Resolved Fluorescence, TR-FRET, Fluorescence Polarization, and Alpha Technology.

Supplier: MP Biomedicals

HEPES is a zwitterionic organic chemical buffering agent.
HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media.

Supplier: Spectrum Chemical Mfg. Corp.

Potassium Biphthalate, BiotechGrade, also known as potassium hydrogen phthalate or KHP, is an acidic salt compound used as a primary standard for acid-base titrations and as a buffering agent. The critical parameters involved are absence of inhibitors such as traces of heavy metals as well as biochemical function tests for enzymes, coenzymes and enzyme substrates.

Supplier: MP Biomedicals

Bis-Tris is a zwitterionic buffer; an amine buffer similar to Tris Hydrochloric acid that exhibits only a very small shift in dissociation constant with respect to temperature. It is structurally analogous to the the Good buffers that were developed to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies.

Supplier: Spectrum Chemical Mfg. Corp.

Sodium Thiosulfate, Pentahydrate, Crystal, BiotechGrade has been used to lessen some of the side effects of cisplatin (a cancer medicine). It is also used with another medicine in the emergency treatment of cyanide poisoning. The critical parameters involved are absence of inhibitors such as traces of heavy metals as well as biochemical function tests for enzymes, coenzymes and enzyme substrates.

Supplier: MP Biomedicals

Sodium chloride is a commonly used chemical which is found widely in nature. It is considered to be an essential nutrient. Excess amounts of sodium chloride can destroy electrolyte balance and cause death in most animals, including humans.
Sodium chloride is used in a wide variety of biochemical applications, including intravenous fluids (normal saline is 0.9% w/v in water 10), density gradients 3,6, a diluent to increase ionic strength in buffers or culture media and in salt-out procedures in the isolation of DNA. It has also been used in high concentrations for preservation of foods since bacteria cannot grow in high salt conditions. A salt-ice mixture in the ratio of approximately 33 g sodium chloride to 100 g ice (at -1°C) will drop in temperature to as low as -21°C, depending on the rate of stirring and the size of the ice chunks.

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Protect and stabilize valuable biochemical proteins and streamline downstream processing with reliable, high-performance sugars.

Supplier: MP Biomedicals

Tweens® are a series of nonionic surfactants derived from sorbitan esters. They are soluble or dispersible in water but differ widely in organic and oil solubilities. Used as oil-in-water emulsifiers in pharmaceuticals, cosmetics, cleaning compounds, etc.
Tween® 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture selective protein extraction growing of tubercule bacilli, and emulsifying and dispersing substances in medicinal and food products. It has little or no activity as an anti-bacterial agent. It has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds. Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
Soluble/miscible in water to give a clear yellow solution; miscible with alcohol, dioxane, and ethyl acetate; practically insoluble in liquid paraffin and fixed oils (such as mineral oil).
Autoclaving of solutions is not recommended. Sterile filtering is suggested with a 0.22 micron filter. Tween® may need to be warmed to about 40 °C and alternated with portions of hot distilled water while being poured through the filter.
Store at Room Temperature (15-30 °C).

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: QUALITY BIOLOGICAL, INC.

Molecular Biology Water is prepared by reverse osmosis, passed through fine carbon, deionized through two resin beds, and serially filtered through 0.1 µm positively charged membranes.

Supplier: VWR

Bradford's reagent, Biotechnology Grade for quantitative protein determination

Supplier: TCI America

[for Biochemical Research]
CAS Number: 9002-98-6
Form: Clear Liquid
Color: Colorless

Supplier: TCI America

[for Biochemical Research]
CAS Number: 6226-79-5
MDL Number: MFCD00003892
Molecular Formula: C22H16N4O13S4
Molecular Weight: 760.55
Purity/Analysis Method: >85.0% (N)
Form: Crystal

Supplier: VWR

A reagent for the preparation of competent cells for transformation.

Supplier: TCI America

[for Biochemical Research]
CAS Number: 63-74-1
MDL Number: MFCD00007939
Molecular Formula: C6H8N2O2S
Molecular Weight: 172.20
Purity/Analysis Method: >99.0% (T)
Form: Crystal
Color: White
Melting point (°C): 166
Lambda max.: 257 nm (H2O)

Supplier: TCI America

[for Biochemical Research]
CAS Number: 123333-70-0
MDL Number: MFCD00064394
Molecular Formula: C6H7NO3S
Molecular Weight: 195.17
Purity/Analysis Method: >98.0% (T)
Form: Crystal
Color: White

Supplier: TCI America

[for Biochemical Research]
CAS Number: 771-97-1
MDL Number: MFCD00004116
Molecular Formula: C10H10N2
Molecular Weight: 158.20
Purity/Analysis Method: >98.0% (GC,T)
Form: Crystal
Melting point (°C): 198
Storage Temperature: 0-10°C

Supplier: Thermo Scientific Chemicals

Liquid

Supplier: Thermo Scientific Chemicals