PureCol® Bovine Collagen Type I
Supplier: Advanced Biomatrix
PureCol® EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator. The product consists of purified Type I collagen at a concentration of 0.5%, (~5 mg/ml), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture.
PureCol® EZ Gel is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions.
PureCol® EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. PureCol® EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.
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TeloCol® Bovine Telocollagen Type I
Supplier: Advanced Biomatrix
TeloCol®-3 bovine collagen solution is derived from an acid extraction process yielding a telopeptide-intact collagen. The pro-peptide regions at both ends of the collagen chain, N- and C-telopeptide regions, are maintained.
TeloCol®-3 collagen is approximately 95% Type I collagen with the remainder being comprised of Type III collagen. Each product includes a bottle containing 50 ml of collagen solution accompanied with a bottle of pre-formulated neutralizing solution for the formation of a collagen gel. This product is supplied at a concentration of approximately 3 mg/ml with the concentration for each specific lot provided on the product label and Certificate of Analysis that is available with the purchase of each product. TeloCol®-3 is sterile filtered and provided in user-friendly packaging for use and storage.
TeloCol®-3 is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.
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TeloCol® Bovine Telocollagen Type I
Supplier: Advanced Biomatrix
TeloCol®-6 bovine collagen solution is derived from an acid extraction process yielding a telopeptide-intact collagen. The pro-peptide regions at both ends of the collagen chain, N- and C-telopeptide regions, are maintained.
TeloCol®-6 collagen is approximately 95% Type I collagen with the remainder being comprised of Type III collagen. Each product includes a bottle containing 50 ml of collagen solution accompanied with a bottle of pre-formulated neutralizing solution for the formation of a collagen gel. This product is supplied at a concentration of approximately 6 mg/ml with the concentration for each specific lot provided on the product label and Certificate of Analysis that is available with the purchase of each product. TeloCol®-6 is sterile filtered and provided in user-friendly packaging for use and storage.
TeloCol®-6 is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.
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Rapid Response™ Plastic Collection Cup with Temperature Strip, BTNX
Supplier: BTNX INC.
The Rapid Response™ Plastic Collection Cup with Temperature Strip is the perfect solution for reliable urine sample collection. With a built-in temperature strip, it ensures that the sample is freshly collected, making it ideal for drug screening purposes.
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SARS-CoV-2 Recombinant Spike Protein, aa16-685 (HEK293-expressed)
Supplier: Stemcell Technologies
SARS-CoV-2 Recombinant Spike Protein, aa16-685 is expressed in HEK293 cells and is one of four structural proteins encoded by the SARS-CoV-2 genome. The Spike Protein plays a key role in attachment to host cells, allowing invasion through clathrin-mediated endocytosis. The Spike Protein can be cleaved by host cell proteases after aa685 to yield the N-terminal S1 subunit and C-terminal S2 region. The S1 subunit is responsible for interacting with the host cell receptor (angiotensin-converting enzyme II) through a receptor-binding domain that is highly conserved with SARS-CoV. The S1 subunit has two conformations: a ‘down’ conformation in which the receptor is inaccessible, and an ‘up’ conformation in which the receptor is accessible. These conformational changes are key for monoclonal antibody drugs and vaccine development. SARS-CoV-2 Recombinant Spike Protein contains a polyhistidine tag at the amino terminus; it also contains a FLAG tag at the carboxy terminus.
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DRG® C-Reactive Protein HS ELISA, DRG International, Inc.
Supplier: DRG International
A high sensitivity enzyme immunoassay for the quantitative determination of C-reactive protein (CRP) concentration in serum.
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QuickSilver™ Buffer Powder
Supplier: Alkali Scientific
Prepare your buffers easily with QuickSilver™ buffer powder, ensuring high-quality solutions for your laboratory experiments.
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Poly-D-lysine 0,1 mg/mL
Supplier: Advanced Biomatrix
Poly-D-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule is resistant to enzymatic degradation and has been used to culture a wide variety of cell types.
The molecular weight of Poly-D-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.
Poly-D-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions. Poly-D-Lysine is supplied in a sterile 5 mg package size.
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EDTA ferric monosodium salt
Supplier: Spectrum Chemical Mfg. Corp.
Ethylenediaminetetraacetic Acid, Iron (III) Sodium Salt or EDTA iron(III) sodium salt and is used to eliminate enzyme inhibition by traces of heavy metals, and to inhibit enzymes that require divalent cations as cofactors.
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Automated Nucleic Acid Purification System MPure-12™, MP Biomedicals
Supplier: MP Biomedicals
MPure-12™ is a bench top automated system for rapid purification of nucleic acids from a wide variety of bio-specimens using magnetic bead separation technology. It has a uniquely designed magnetic bead processing chamber and is fully integrated with easy to use pre-packaged reagent kits.
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Whatman™ 903 Proteinsaver Sample Collection Card, Whatman products (Cytiva)
Supplier: Cytiva
Whatman 903 sample collection cards are designed for the collection of biological fluids
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Whatman™ 903 Proteinsaver Snap-Apart Sample Collection Card, Whatman products (Cytiva)
Supplier: Cytiva
903 sample collection card with four sample spots.
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Poly-L-Lysine
Supplier: Advanced Biomatrix
Poly-L-Lysine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of Lysine. Poly-L-Lysine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule has been used to culture a wide variety of cell types. Certain cell types secrete proteases, which can digest Poly-L-Lysine. For those cell types, Poly-D-Lysine 000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.
Poly-L-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions. This product is supplied in a sterile 50 ml package size at a concentration of 0.1 mg/ml.
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ImageXpress® Micro Confocal High-Content Imaging System, Molecular Devices
Supplier: Molecular Devices
Explore new dimensions with the confocal system for complex biology. The ImageXpress® Micro Confocal High-Content Imaging System enables users to expand the boundaries of research with the ultimate combination of speed, sensitivity, and flexibility in a turn-key solution.
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Bovine Fibrinogen (from Plasma), MP Biomedicals
Supplier: MP Biomedicals
Background Information
Fibrinogen is a blood protein that is involved in clotting and is converted to fibrin by thrombin. Fibrinogen has an approximate molecular weight of 340 kDa. It consists of three non-identical pairs of disulfidebonded chains. The α-chain has an approximate molecular weight of 63.5 kDa, the β-chain 56 kDa, and the γ-chain 47 kDa. At the amino termini, the three chains are connected in a dimeric disulfide knot (DSK). A second DSK occurs later in the molecule. Fibrinogen has approximately 4% carbohydrate content.
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PureCol® Bovine Collagen
Supplier: Advanced Biomatrix
PureCol® collagen is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available. PureCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.
PureCol® collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.
This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.
PureCol® collagen is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol®, lyophilized form is not recommended for the formation of a solid gel. PureCol® collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.
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Mouse Recombinant PDGF-BB
Supplier: Stemcell Technologies
The platelet-derived growth factor (PDGF) family has five heparin-binding members that assemble into four homodimers (PDGF-AA, PDGF-BB, PDGF-CC, and PDGF-DD) and one heterodimer (PDGF-AB; Fretto et al.; Li and Eriksson). PDGF signals through the receptor tyrosine kinases PDGFRα and PDGFRβ. It has been shown that PDGF-induced migration involves signaling pathways involving MEK/ERK, EGFR, Src, and PI3K/AKT (Kim et al.). PDGF is a potent mitogen for cells of mesenchymal origin, such as fibroblasts, glial cells, and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto et al.; Sachinidis et al.). PDGF-BB is secreted by osteoblasts to induce mesenchymal stem cell migration and angiogenesis. It has also been shown that PDGF-BB is secreted by preosteoclasts during bone modeling and remodeling to induce angiogenesis and thus proper osteogenesis (Xie et al.).
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Multi-Syringe Programmable Syringe Pump, Braintree Scientific
Supplier: BRAINTREE SCIENTIFIC INC MS
Multi-syringe programmable syringe pump built for automation - Operates stand-alone or from a computer. Infusion and withdrawal, set a single pumping rate, set a dispensing volume, or program up to 41 pumping phases that change pumping rates, set dispensing volumes, insert pauses, control and respond to external signals, sound the buzzer.
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Anti-GLRA1 Rabbit Polyclonal Antibody
Supplier: Prosci
Gamma-amGlycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission. The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin. Gene targeting in mice showed that gephyrin is required for synaptic clustering of glycine receptors in spinal cord. The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins. These receptors have also been extremely useful in studies of synaptic clustering of receptors. During postnatal motoneuron development, the glycine receptor alpha subunit changes from alpha2 (fetal) to alpha1 (adult).
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BCR-ABL MUTATION DETECTION KIT
Supplier: HUMAN HEALTH DIAGNOSTICS MS
BCR-ABL MUTATION DETECTION KIT
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FibriCol® Bovine Collagen Type I
Supplier: Advanced Biomatrix
FibriCol® collagen is known as the standard of all collagens for purity (>99% collagen content), functionality, and the most native-like collagen available. FibriCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.
FibriCol® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography.
FibriCol® collagen is supplied at approximately a 10 mg/mL concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. FibriCol® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.
FibriCol® collagen is ideal for applications where high concentrations of collagen are needed. FibriCol® collagen is provided in a 20 mL volume and is contained in user-friendly packaging for use and storage. FibriCol® is sterile filtered and is supplied as a ready to use solution.
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Mouse Urocortin 2 ELISA Assay Kit, Eagle Biosciences
Supplier: Eagle Biosciences
Mouse Urocortin 2 ELISA is used to detect urocortin 2 in mouse plasma & serum samples.
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L-Arginine hydrochloride
Supplier: Spectrum Chemical Mfg. Corp.
L-Arginine Hydrochloride is an alpha-amino acid that is a semi-essential amino acid. Ungraded products supplied by Spectrum are indicative of a grade suitable for general industrial use and typically are not suitable for consumption.
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Nutragen® Bovine Collagen
Supplier: Advanced Biomatrix
Nutragen® collagen is known as the standard of all collagens for purity (>99% collagen content), functionality, and the most native-like collagen available. Nutragen® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.
Nutragen® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography.
Nutragen® collagen is supplied at approximately a 6 mg/mL concentration to provide an extremely firm gel. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. Nutragen® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.
Nutragen® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel. Nutragen® collagen is provided in a 50 mL volume and is contained in user-friendly packaging for use and storage. Nutragen® is sterile filtered and is supplied as a ready to use solution.
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FailSafe™ PCR Systems, PCR Optimization Kits, Biosearch Technologies
Supplier: Lucigen
Dependable, consistent high-fidelity PCR results with every target DNA template, every time
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Optifit Pipette Tips
Supplier: Sartorius
Sartorius optifit pipette tips are an excellent choice for various laboratories and pipetting tasks with their wide range of packaging and purity options. These tips are non-filtered and designed for best possible fitting and sealing on Sartorius pipettes to secure the highest possible accuracy, precision, and reproducibility.
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Bovine Collagen Type I (Methacrylated)
Supplier: Advanced Biomatrix
Advanced BioMatrix offers PhotoCol®, a purified methacrylated Type I bovine collagen kit. PhotoCol® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.
The PhotoCol® kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents in the kit.
The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the e-amines groups of the lysine residues as well as the a-amines groups on the N-termini. Approximately 40% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content. The collagen starting material was isolated from a closed herd and purified using controlled manufacturing processes. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml.The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture.The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included) which allows UV crosslinking of the collagen at 365 nm.
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NxSeq® UltraLow DNA Library Kit and Indexing Kits, NGS DNA Library Prep, Illumina-compatible, Biosearch Technologies
Supplier: Lucigen
Generate highly complex, Illumina-compatible DNA fragment libraries with ≥50 pg of DNA with this quick and easy kit
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MagneSil Blood Genomic, Max Yield System, 1 x 96 preps, Promega
Supplier: Promega Corporation
MagneSil Blood Genomic, Max Yield System provides automated high-throughput DNA purification on the Beckman Coulter Biomek FX using MagneSil Paramagnetic Particle technology.
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Lentiviral Packaging Plasmid Mix, Cellecta
Supplier: CELLECTA, INC. MS
Lentiviral packaging plasmid mix allows the production of pseudoviral particles when mixed with second- or third-generation lentiviral vectors.