Ward's® Screening Hemoglobin for Sickle Cell Lab Activity

Identify a protein defect using agarose electrophoresis.

• Students Simulate Protein Electrophoresis
• Materials for Eight Lab Groups
• Time Required: ~90 Minute

In an attempt to determine the presence of the sickle-cell anemia trait, students will use agarose electrophoresis to separate several simulated hemoglobin protein samples.

They will learn to identify and differentiate between normal hemoglobin and sickle-cell hemoglobin. They will also learn to identify those who are heterozygous for the sickle-cell gene.

 : An agarose electrophoresis chamber, power supply, and 10 μL micropipets, required, are available separately.

 : Eight gels are included in the kit as well as a teacher’s guide and student copy master.