Edvotek® Reeling In The Truth: Identifying Red Snapper Using Genetic Analysis

Is what you are buying what you are getting?

• Extract DNA from simulated fish samples and amplify specific gene regions. Understand how conserved gene regions can be used to identify species and study genetic diversity Explore DNA barcoding, a critical tool in species identification

In this experiment, students will explore the genetic diversity of fish species in a simulated DNA barcoding experiment. Students will extract DNA from simulated fish samples and use PCRto amplify three gene regions that are conserved between species.

Ordering information: You need: DNA electrophoresis, thermal cycler, micropipettes: 5 to 50 µl, UV or blue light transilluminator, white light box (optional - use if staining with FlashBlue™™ stain), microcentrifuge, and microwave or hot plate.

Delivery information: Includes instructions, PCREdvoBeads, LyphoPrimer™ Mix, EdvoQuick DNA Ladder, Red Snapper Template, DNA Templates #1 - 3, TE Buffer, Universal DNA Buffer, Proteinase K, rice paper for simulated fish samples, Ultra-Spec Agarose, 50× electrophoresis buffer, FlashBlue™ DNA stain, SYBR™ Safe, 1.5 ml microcentrifuge tubes, 1.5 ml screw-cap tubes, 0.2 ml PCR tubes, petri plates.