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Western Blot Analysis Kit, Edvotek

Western blot analysis kit, 6
Catalog # 470120-900
Western Blot Analysis Kit, Edvotek
Catalog # 470120-900
Supplier:  Edvotek
Western Blot Analysis Kit, Edvotek
Western Blot Analysis Kit, Edvotek

Western blot analysis kit, 6

Specifications
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Specifications
Price
$325.00
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  • Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
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Product Details & Documents

Protein identification is based on both antibody reactions and antigens in this Western Blot activity.

  • Materials for Six Blots Included
  • Instructions with Background Information
  • Students Research Protein Identification

A denaturing SDS polyacrylamide gel separates the proteins, which are transferred or blotted to a nylon membrane. The membrane is then exposed sequentially to solutions containing the primary antibody, followed by a secondary antibody that is coupled to an enzyme.

The membrane is soaked in a substrate solution to develop the color reaction, which results in identifying the antigen as a band, and the molecular weights of the visible bands are measured using pre-stained protein markers.

Ordering information: This kit contains negative lyophilized control BSA, high and low concentration (lyophilized); an anti-BSA protein antibody; secondary antibody conjugate; hydrogen peroxide; peroxide co-substrate, 10X; blocking buffer; PBS buffer; pre-stained protein standard marker (lyophilized); tris-glycine-SDS electrophoresis buffer; powdered milk; and instructions with background information. The kit requires but does not include three polyacrylamide gels, MV-10 vertical gel electrophoresis apparatus, DC power supply, automatic micropipet, fine micropipet tips, glacial acetic acid, methanol, and distilled or deionized water.

Delivery information: This kit is shipped ambient. Store lyophilized proteins at room temperature.

Specifications
  • Description:
    Western blot analysis kit
  • No. of lab groups:
    6
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