Protease Assay Kit, G-Biosciences

The Protease Assay Kit is designed for the quantitative determination of proteases present in protein sample, using a dye-labeled protein substrate

Chemically stabilized Trypsin (MSG-Trypsin™) is supplied with the kit as a general protease standard; however, other specific protease standards can also be used. MSG-Trypsin™ is an ultra-pure trypsin from bovine pancreas, modified by methylation followed by TPCK treatment and is extremely resistant to autolysis. The kit components are sufficient for 50 assays in a microtiter plate format or 0.5ml assay tubes.

Accessories: Resorufin-Casein Protease Substrate (#786-321): A colorimetric substrate that when treated with proteases releases resorufin that has an absorbance of 570nm. Supplied lyophilized. FITC-Casein Protease Substrate (#786-322): A fluorescent (fluorescein isothiocyanate (FITC)) substrate that when treated with proteases releases FITC that has an excitation at 485nm and emission at 535nm. Supplied lyophilized.

The proteases present in the sample of interest will digest the protein substrate and release dye labeled peptides. The absorbance of the dye-labeled peptide is measured at 570nm for determination of protease activity.

Ordering information: Accessories not included.