miniPCR® BioBits® Central Dogma
About this item
Illuminate transcription and translation with the BioBits® cell-free system in this innovative classroom-sized kit!
- Techniques: Micropipetting, fluorescence detection, cell-free technology
- Topics: Central dogma of molecular biology, transcription and translation, protein synthesis, gene expression, fluorescence, biotechnology, synthetic biology
- Time required: 30 minutes during 1 class period, followed by 10 minutes for observation 8 - 72 hours later
- Level: General high school through college
BioBits® pellets are tiny molecular factories that can create proteins without the need for cell culture. When dry, BioBits® pellets are dormant, but they can be activated by simply adding water. Researchers have been using cell-free reactions in their laboratories for years, with applications ranging from novel therapeutic discovery to field diagnostics. Now the BioBits® cell-free system makes this cutting-edge technology accessible to anyone interested in learning molecular biology. With minimal equipment requirements and a quick and straightforward protocol, students will use the BioBits® system to visualize the flow of genetic information and monitor transcription and translation in real-time through fluorescence. This activity serves as an excellent interactive tool for learning molecular biology’s central dogma and exposes students to cutting-edge synthetic biology.
Required equipment (one per lab group):
- Micropipettes and tips
- P51™ Molecular Fluorescence Viewer or other blue light transilluminator with an orange filter (460 - 480 nm, e.g. blueBox™ or blueGel™)
Storage notes:
- Reagents require freezer storage
- Reagents are stable for 6 months when stored in the freezer
- If a freezer is not available, BioBits® pellets are viable for three months when stored in a refrigerator
Delivery information: The kit contains the following reagents for 8 lab groups: 32 BioBits® pellets in 8-tube PCR strips; DNA sample A; DNA sample B; nuclease-free water; Kanamycin.
2 options available below
- Return Policy
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
Product Details
Illuminate transcription and translation with the BioBits® cell-free system in this innovative classroom-sized kit!
- Techniques: Micropipetting, fluorescence detection, cell-free technology
- Topics: Central dogma of molecular biology, transcription and translation, protein synthesis, gene expression, fluorescence, biotechnology, synthetic biology
- Time required: 30 minutes during 1 class period, followed by 10 minutes for observation 8 - 72 hours later
- Level: General high school through college
BioBits® pellets are tiny molecular factories that can create proteins without the need for cell culture. When dry, BioBits® pellets are dormant, but they can be activated by simply adding water. Researchers have been using cell-free reactions in their laboratories for years, with applications ranging from novel therapeutic discovery to field diagnostics. Now the BioBits® cell-free system makes this cutting-edge technology accessible to anyone interested in learning molecular biology. With minimal equipment requirements and a quick and straightforward protocol, students will use the BioBits® system to visualize the flow of genetic information and monitor transcription and translation in real-time through fluorescence. This activity serves as an excellent interactive tool for learning molecular biology’s central dogma and exposes students to cutting-edge synthetic biology.
Required equipment (one per lab group):
- Micropipettes and tips
- P51™ Molecular Fluorescence Viewer or other blue light transilluminator with an orange filter (460 - 480 nm, e.g. blueBox™ or blueGel™)
Storage notes:
- Reagents require freezer storage
- Reagents are stable for 6 months when stored in the freezer
- If a freezer is not available, BioBits® pellets are viable for three months when stored in a refrigerator
Delivery information: The kit contains the following reagents for 8 lab groups: 32 BioBits® pellets in 8-tube PCR strips; DNA sample A; DNA sample B; nuclease-free water; Kanamycin.
Product Family Options
Product Information
- No. of lab groupsAvailabilityPrice
- 470351-982miniPCR® BioBits®: Central Dogma (classroom kit)Supplier Number: KT-1910-01Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 470352-056miniPCR® BioBits®: Central Dogma minikit refillSupplier Number: KT-1910-02Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.