Short Fragment Eliminator Expansion

An expansion pack designed for size selection of high molecular weight DNA samples to progressively deplete short fragments of <25 kb.

• Size select to progressively deplete short fragments (<25 kb) prior to sequencing library preparation
• Enrich sequencing libraries for long fragments without using any external third-party reagents

The Short Fragment Eliminator (EXP-SFE001) Expansion pack is recommended for users who want to size select high molecular-weight (HMW) gDNA to deplete short fragments (<25 kb) using size selective precipitation. The kit contains Short Fragment Eliminator (SFE) buffer which progressively removes fragments up to 25 kb, with fragments under 10 kb being almost completely removed in a quick and simple workflow.

Note: This kit is designed to remove short DNA fragments and requires samples to be predominantly made up of HMW DNA (>48 kb). If there are large amounts of short fragments in your sample, this will result in a low yield at the end of the prep and a low sequencing output.

Delivery information: This kit is stable during shipping for seven days at temperatures up to 25 °C. Upon receipt, please store the kit under the recommended conditions specified on the kit label. Oxford Nanopore Technologies deem the useful life of the product to be three months from receipt by the customer.

Caution: Oxford Nanopore Technologies products are not intended for use for health assessment or to diagnose, treat, mitigate, cure, or prevent any disease or condition.