pCI-neo Mammalian Expression Vector, 20 µg, Promega

Contains a neomycin resistance gene and the CMV immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. Transfected cells can be selected with G-418.

• Constitutively Express Cloned Insert for Transient or Stable Transfection
• Select for stably transfected cells with neomycin phosphotransferase gene
• Increase the steady-state level of RNA with the late SV40 polyadenylation signal approximately fivefold more than the early SV40 polyadenylation signal
• Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate ssDNA in E. coli using the f1 origin of replication

The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. This vector also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418.