You searched for: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

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Pierce™ DAB Substrate Kit, Thermo Scientific

Supplier: Invitrogen

The Thermo Scientific Pierce DAB Substrate Kit enables chromogenic detection of horseradish peroxidase (HRP) activity based the action of 3,3'-diaminobenzidine (DAB) in Western blot and tissue staining methods.

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Afyon SDS-PAGE Sample Preparation Kit, Advansta

Supplier: ADVANSTA INC MS

Kit for fast, easy concentration and purification of samples for SDS-PAGE.

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WesternBright Sirius HRP Substrate, Advansta

Supplier: ADVANSTA INC MS

Sirius is the most sensitive chemiluminescent substrate from Advansta.

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Detergents and Blocking Reagents for Blotting, Bio-Rad

Supplier: Bio-Rad

Bio-Rad offers blotting-grade non-fat milk, gelatin, phosphate buffered saline with 1% casein, Tris buffered saline with 1% casein, Tween 20, and 20% SDS

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Pierce QuantaBlu™ Fluorogenic Peroxidase Substrates, Thermo Scientific

Supplier: Invitrogen

The substrate generates a blue fluorescent product upon reaction with peroxidase and does not photobleach.

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WesternBright Quantum HRP Substrate, Advansta

Supplier: ADVANSTA INC MS

Advansta's Quantum is ideal for quantitative chemiluminescent Western blotting.

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Pierce™ Restore™ PLUS Western Blot Stripping Buffer, Thermo Scientific

Supplier: Invitrogen

An advanced formula for removing high-affinity bound primary and secondary antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

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Avidin/Biotin Blocking Kit, Vector Laboratories

Supplier: Vector Laboratories

Avidin/Biotin Blocking Kit blocks all endogenous biotin, biotin receptors, and avidin binding sites present in tissues.

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Pierce™ NBT Substrate Powder (nitro-blue tetrazolium chloride), Thermo Scientific

Supplier: Invitrogen

NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt

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Pierce™ Metal Enhanced DAB Substrate Kit, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce™ Metal-Enhanced DAB Substrate Kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate.

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SuperSignal™ West Pico Complete Mouse IgG Detection Kit, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific SuperSignal West Pico Complete IgG Detection Kit is a a kit containing goat anti-mouse or anti-rabbit secondary antibody HRP conjugate for the detection of mouse or rabbit primary antibody

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Precision Plus Protein™ Unstained Standards, with Strep-tag, Bio-Rad

Supplier: Bio-Rad

Precision Plus Protein™ Unstained Standards contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD, of which three are high-intensity reference bands (25, 50, and 75 kD)

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HRP and AP Antibody Conjugates, Bio-Rad

Supplier: Bio-Rad

Bio-Rad Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP) Secondary Antibody Conjugates are engineered for use with blot detection protocols including colorimetric and chemiluminescent.