You searched for: Protein Extraction Reagents
Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.
ProteaseMAX™ Surfactant, Trypsin Enhancer, Promega
Supplier: Promega Corporation
ProteaseMAX™ surfactant, trypsin enhancer enhances the enzymatic performance of trypsin, chymotrypsin and Lys-C in digestion reactions.
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Trypsin/Lys-C Mix, MS Grade, Promega
Supplier: Promega Corporation
Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.
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Lumi Super Tracker™ Soluble/ELISA Chemiluminescent Peroxidase Substrate, ImmunO4
Supplier: Immuno4 LLC
Developed specifically for ELISA and In-Vitro Diagnostic applications requiring ultra sensitivity with exceptional signal to noise ratios.
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Iminobiotin Resin for Isolation of Avidin, Streptavidin and Neutravidin Complexes, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose
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Q-sep® QuEChERS Extraction Salts, Restek
Supplier: Restek
QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.
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Factor Xa Protease, New England Biolabs
Supplier: New England Biolabs (NEB)
Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg
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Protein Reducing Agents, G-Biosciences
Supplier: G-Biosciences
A water soluble, odorless, non-toxic and stable protein reductant, G-Biosciences' Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol)
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ImmunoHistoZyme™, Ready-to-Use, Electron Microscopy Sciences
Supplier: Electron Microscopy Sciences
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
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Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific
Supplier: Invitrogen
Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling
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VersaLyse™ RBC Lysing Solution, Beckman Coulter
Supplier: Beckman Coulter
VersaLyse lysing solution is intended for the lysis of red blood cells in the preparation of biological samples for flow cytometry analysis.
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Pierce Imject™ Maleimide-Activated Ovalbumin, Thermo Scientific
Supplier: Invitrogen
Imject™ Maleimide-activated ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.
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Pierce™ Immobilized Papain (Agarose Resin), Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Immobilized Papain consists of a cysteine-endopeptidase (papain) that has been immobilized onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.
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Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.
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Pierce™ Endoproteinase Chymotrypsin Protease (TLCK treated), MS Grade, Thermo Scientific
Supplier: Invitrogen
Chymotrypsin Protease, MS Grade, is a purified native endoproteinase validated for maximum activity and selectivity in proteomic and used in in-solution digestions and in-gel digestion of proteins from from 1-D or 2-D gels. Store at -20˚C in a nonfrost-free freezer.
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Pierce™ Endoproteinase Asp-N Protease, MS Grade, Thermo Scientific
Supplier: Invitrogen
Asp-N Protease, MS Grade, is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications. Store at -20˚C in a nonfrost-free freezer.
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Pierce™ Immobilized Pepsin (Agarose Resin), Thermo Scientific
Supplier: Invitrogen
Immobilized Pepsin agarose consists of an acidic endopeptidase that has been immobilized onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.
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Pierce™ Surfact-Amps™ Detergent Solutions, Thermo Scientific
Supplier: Invitrogen
Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques.
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Pierce™ Endoproteinase Lys-C Protease, MS Grade, Thermo Scientific
Supplier: Invitrogen
Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
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ReadyPrep Reagents for Extraction and Solubilization, Bio-Rad
Supplier: Bio-Rad
A number of ReadyPrep kits are available for total protein or targeted protein extraction (fractionation)
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Pierce™ Endoproteinase Glu-C Protease, MS Grade, Thermo Scientific
Supplier: Invitrogen
Glu-C Protease, MS Grade, is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications, in-solution digestion of proteins and in-gel digestion of proteins. Store at -20 ˚C in a nonfrost-free freezer.
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Pierce™ Micrococcal Nuclease Solution, Thermo Scientific
Supplier: Invitrogen
The Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.