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51 results for Protein Extraction Reagents

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Protein Extraction Reagents

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

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BugBuster® HT Protein Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster® HT Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E

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Tissue Protein Extraction Reagent, Abcam

Supplier: Abcam

This product is manufactured by BioVision, an Abcam company and was previously called 8002 EZLys™ Tissue Protein Extraction Reagent.

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BugBuster® Protein Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster® Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E.coli, resulting in the liberation of soluble protein.

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BugBuster® 10X Protein Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster 10X is a concentrated formulation of the proprietary detergents employed in BugBuster without the addition of salts or buffer components.

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PopCulture® Reagent, MilliporeSigma

Supplier: MilliporeSigma

PopCulture Reagent* is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium.

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Trypsin Antigen Retrieval Kit, Abcam

Supplier: Abcam

Trypsin Antigen Retrieval Kit ab259256 is used for enzyme-based antigen retrieval.

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CHO/K1 Whole Cell Lysate, Rockland Immunochemical

Supplier: Rockland Immunochemical

Ready-to-use whole cell lysates are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility.

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Pierce™ Trypsin Protease, MS Grade, Thermo Scientific

Pierce™ Trypsin Protease, MS Grade, Thermo Scientific

Supplier: Invitrogen

Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

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Lysing Matrix Z, MP Biomedicals

Lysing Matrix Z, MP Biomedicals

Supplier: MP Biomedicals

Lysing Matrix Z is used for breaking tough plant and animal samples.

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Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific

Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific

Supplier: Invitrogen

The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.

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Trypsin Antigen Retrieval Solution, Abcam

Supplier: Abcam

Trypsin-based antigen retrieval solution for Protease-Induced Epitope Retrieval (PIER) (ab970).

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FLUOR DE LYS® Developer II Concentrate, Enzo Life Sciences

FLUOR DE LYS® Developer II Concentrate, Enzo Life Sciences

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates.

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Pierce™ Detergent Removal Spin Column, Thermo Scientific

Pierce™ Detergent Removal Spin Column, Thermo Scientific

Supplier: Invitrogen

Pierce™ Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10 µL to 1 mL samples with minimal sample loss.

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SMART Digest™ Protein Digests Kits

SMART Digest™ Protein Digests Kits

Supplier: Thermo Scientific

Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits.

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Universal HIER antigen retrieval reagent (10X), Abcam

Supplier: Abcam

Universal HIER antigen retrieval reagent (10X) is a heat-induced epitope retrieval (HIER) solution that is compatible with virtually all antibodies and eliminates the need for multiple buffers, including citrate buffer, EDTA or high pH Tris buffers. - Cited in over 20 publications - Colour-coded pH indicator for accuracy - Superior pH stability at high temperatures - Non-toxic, odorless, and azide-free - Optimized for FFPE tissue antigen retrieval

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VersaLyse™ Lysing Solution, 100 Tests, RUO

Supplier: Beckman Coulter

VersaLyse Lysing solution is a reagent used to lyse red blood cells from any biological fluid and, in particular, to lyse erythrocytes from whole blood. VersaLyse is a highly specific, very gentle lysing solution, suitable for applications such as immunophenotyping, cell sorting, activation or cell culture. It is designed for use in flow cytometry and facilitates obtaining highly discriminative scattergrams whatever the type of flow cytometer used. VersaLyse is a versatile reagent which may be used in lyse/wash, wash/lyse and lyse/no-wash procedures with undiluted or diluted samples. The histograms are dual-parameter light scatter histograms (FS versus SS) of a normal K3EDTA anticoagulated blood sample lysed with VersaLyse Lysing Solution (No-wash procedure).

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BugBuster® (primary amine-free) Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilization or cross-linking.

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HistoReveal (15ml), Abcam

Supplier: Abcam

Proteolytic antigen retrieval (PIER) reagent HistoReveal (ab103720).

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FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences

FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.).

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FASP Protein Digestion Kit (FFPE), Abcam

Supplier: Abcam

FASP Protein Digestion Kit (FFPE) (ab270543) allows unbiased extraction, complete proteome solubilization, and highly efficient digestion of FFPE tissue.

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Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific

Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.

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Lysonase™ Bioprocessing Reagent, EMD Millipore

Supplier: MilliporeSigma

Lysonase™ bioprocessing reagent is an optimized, ready-to-use blend of rLysozyme™ solution and benzonase nuclease.

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NP-40 Alternative, MilliporeSigma

Supplier: MilliporeSigma

NP-40 alternative is a non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis.

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PROTEIN GRADE® Detergent, C12E9, Calbiochem®, MilliporeSigma

Supplier: MilliporeSigma

A non-ionic detergent used for the solubilization of membrane-bound proteins in their native state.

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B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific

B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific

Supplier: Invitrogen

B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.

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Lumi Super Tracker™ Membrane/Blotting Chemiluminescent Peroxidase Substrate, ImmunO4

Supplier: Immuno4 LLC

Developed specifically for membrane/blotting applications requiring ultra sensitivity with exceptional signal to noise ratios.

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Lightning in a Bottle™, Chemiluminescent Membrane/Blotting Peroxidase Substrate

Supplier: Immuno4 LLC

Developed specifically as a ready to use reagent for Blotting applications requiring ultra sensitivity with exceptional signal to noise ratios

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Biotin Resin for Isolation of Avidin, Streptavidin & Neutravidin Coupled Molecules, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein.

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Pepsin Reagent, Ready To Use, Electron Microscopy Science

Supplier: Electron Microscopy Sciences

Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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Pronase Reagent, Ready To Use, Electron Microscopy Science

Supplier: Electron Microscopy Sciences

Pronase is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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