You searched for: Cloning Kits and Ligation

Supplier: ReVacc Scientific

This Seamless Assembly Master Mix (2X) uses Gibson cloning principle to allow efficiently assembly of multiple DNA fragments, regardless of fragment length or end compatibility. The insert and the linearized vector need the overlapped sequences of 15 bp - 20 bp on both 5' - and 3' -end, respectively. A positive control with the mixture of insert and linearized vector is included.

Supplier: New England Biolabs (NEB)

Taq DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA

Supplier: New England Biolabs (NEB)

EnGen® Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen Spy Cas9 NLS from streptococcus pyogenes with reduced non-specific DNA cleavage.

Supplier: Promega Corporation

For ligation of DNA ends, mutagenesis and cDNA cloning

Supplier: New England Biolabs (NEB)

Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, truncated K227Q (T4 Rnl2tr K227Q) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Supplier: New England Biolabs (NEB)

A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase.

Supplier: Intact Genomics

T4 DNA ligase displays significantly higher ligation efficiency than the nearest competitor.

Supplier: Intact Genomics

Intact Genomics propriety Ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site.

Supplier: Intact Genomics

Taq ligase is thermostable DNA ligase from the thermophilic bacterium Thermus aquaticus cloned to E. coli cells.

Supplier: Intact Genomics

Propriety ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR products to any linearized expression vector at any site.

Supplier: Intact Genomics

T4 DNA ligase displays significantly higher ligation efficiency than the nearest competitor.