You searched for: Electrophoresis Buffers
The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.
Blocking Buffer for Fluorescent Western Blotting, Rockland Immunochemicals
Supplier: Rockland Immunochemical
This blocking buffer is specifically formulated to achieve superior reproducible western blotting images using fluorescent systems.
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Blocking Buffer Sampler Kit, Rockland Immunochemicals
Supplier: Rockland Immunochemical
Blocking Buffer Sampler Kit for ELISA, immunoassays, and western blot
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10X PBS pH 7.2
Supplier: Rockland Immunochemical
10X Phosphate buffered saline (PBS) pH 7.2
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Phosphate Buffered Saline, 0.01 Molar, pH 7.40 at 25°C, RICCA Chemical Company
Supplier: Ricca Chemical
Specifications
Appearance: Colorless liquid
Assay (vs. Silver Nitrate/Potassium Chromate): 0.89-0.91 % (w/v)
pH at 25°C (Method: SQCP027, SQCP033): 7.38-7.42
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Tris-Acetate-EDTA (TAE) 50X, Rockland Immunochemicals
Supplier: Rockland Immunochemical
Tris-Acetate-EDTA (TAE) is a buffer solution containing a mixture of Tris base, acetic acid and EDTA.
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SSC, 20X Buffer, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
SSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridization
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TAE Buffers, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
Tris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run.
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MOPS/SDS, 20X, Running Buffer, Teknova
Supplier: Teknova
MOPS/SDS running buffer is designed for protein electrophoresis.
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Citrate-Buffered Saline (20X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Saline-sodium citrate (SSC) buffer.
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Blocking Buffers, Rockland Immunochemicals
Supplier: Rockland Immunochemical
Use BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk
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Protein Block, Abcam
Supplier: Abcam
Protein block is a serum-free blocking buffer designed to reduce non-specific background staining in IHC, ELISA, and western blot (WB). - Cited in over 90 publications - No need to match species for secondary antibodies, reducing animal serum handling - Ready-to-use – no mixing or dilution required
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Borate Saline Buffered (BSB), Rockland Immunochemicals
Supplier: Rockland Immunochemical
Borate Saline Buffered (BSB) is commonly used in coating procedures for ELISA.
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Pierce™ Fast Wash Buffer, 10X, Thermo Scientific
Supplier: Invitrogen
Pierce Fast Wash Buffer, 10X, for use with Pierce Fast Blocking Buffer.
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CRITERION™ Phosphate Buffer, pH 7.2
Supplier: Hardy Diagnostics
CRITERION™ Agar, bacteriological grade is used as a solidifying agent in the preparation of solid and semi-solid culture media.
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Blue Protein Loading Dye, New England Biolabs
Supplier: New England Biolabs (NEB)
The Blue Loading Buffer Pack contains 3X Blue Loading Buffer (8ml) and 30X Reducing Agent (1ml).
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Advansta Buffer Pouches, Tris-Glycine-SDS
Supplier: Advansta
Convenient, pure, fast buffer preparation
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Immunoassay Blocking (BSA Free)
Supplier: Abcam
Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.
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PBS and TBS buffers, Rockland
Supplier: Rockland Immunochemical
Phosphate buffered and Tris buffered saline are suitable for multiple applications including biological diluent buffer for antibodies or other biologics
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Blocking Solution, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk to reduce the background. Nonfat dried milk is often preferred as it is inexpensive and widely available. However, milk proteins are not compatible with all detection labels, so care must be taken to choose the appropriate blocking solution.
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PreMixed Running Buffers, IBI Scientific
Supplier: IBI Scientific
IBI provides a variety of premixed buffers in various configurations
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Express Transfer Buffer
Supplier: Rockland Immunochemical
Express Transfer Buffer
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Antibody Blocker/Diluent, Enzo Life Sciences
Supplier: Enzo Life Sciences
Ready-to-use antibody blocking buffer and diluent, for use with POLYVIEW® Plus detection reagents.
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TBS 10X Buffer Solution, pH 7.4, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques.
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Pierce™ Blocking Buffer, Protein Free, Thermo Scientific
Supplier: Invitrogen
These protein-free blocking buffers are either a PBS or a TBS formulation of a non protein compound that provides effective blocking for membrane based and plate based protein detection methods, resulting in extremely low background.
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Pierce™ Fast Blocking Buffe, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Fast Blocking Buffer effectively and reliably blocks Western blots in just five minutes to provide low-background results and speed up traditional blotting protocols.
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1X TBS pH 7.8
Supplier: Rockland Immunochemical
1X Tris buffered saline (TBS) pH 7.8
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1X PBS Buffer with 1% Casein
Supplier: Rockland Immunochemical
Phosphate buffered saline with casein is suitable for multiple applications including biological diluent buffer for antibodies or other biologics.
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PBST 10X
Supplier: Rockland Immunochemical
Phosphate Buffer Saline-Tween (PBST) 10X