You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
ADP-Glo Kinase Assay + AKT1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human AKT1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT1/PKB alpha is a serine/threonine kinase that belongs to the AKT family.
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ADP-Glo Kinase Assay + HER4 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human HER4 (amino acids 682-993) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. HER4 or ERBB4 is a transmembrane receptor tyrosine kinase that belongs to the epidermal growth factor receptor family.
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ADP-Glo Kinase Assay + TRKA Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human TRKA (amino acids 440-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TRKA is a member of the trk proto-oncogene family and encodes a 140kDa membrane-spanning protein tyrosine kinase.
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ADP-Glo Kinase Assay + EPHA1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant mouse EPHA1 (amino acids 569-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EPHA1 is a member of the Eph family of receptor tyrosine kinases that have been implicated in mediating developmental events.
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ADP-Glo Kinase Assay + CAMK4 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human CAMK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CAMK4 is a multifunctional serine/threonine protein kinase and a member of Ca2+/calmodulin-dependent protein kinase family.
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ADP-Glo Kinase Assay + p70S6K Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human p70S6K was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues.
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ADP-Glo Kinase Assay + PKC zeta Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PKC zeta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC zeta is an atypical isoform of the PKC family.
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ADP-Glo Kinase Assay + PKC delta Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PKC delta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C delta is a member of the protein kinase C (PKC) family of serine-threonine kinases.
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ADP-Glo Kinase Assay + CDK2/CyclinA2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human CDK2 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed.
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ADP-Glo Kinase Assay + SYK Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human SYK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SYK is a non-receptor protein tyrosine kinase that is widely expressed in hematopoietic cells.
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ADP-Glo Kinase Assay + FAK Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human FAK was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. FAK is involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix.
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ADP-Glo Kinase Assay + EGFR Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human EGFR (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
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ADP-Glo Kinase Assay + RSK2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human RSK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RSK2 is a member of the RSK (ribosomal S6 kinase) family that are growth factor-regulated serine-threonine kinases.
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ADP-Glo Kinase Assay + HER2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human HER2 (amino acids 676-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. HER2 gene encodes a cell-surface glycoprotein tyrosine kinase receptor with extensive homology to epidermal growth factor receptor.
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ADP-Glo Kinase Assay + PDK1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PDK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2.
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ADP-Glo Kinase Assay + PKC gamma Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PKC gamma was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC gamma is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
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Phospholipase D, Streptomyces chromofuscus, Enzyme Assay, MilliporeSigma
Supplier: MilliporeSigma
Native phospholipase D from Streptomyces chromofuscus
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ADP-Glo Kinase Assay + CAMK2 gamma Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human CAMK2 gamma (C-terminal truncation) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. CAMK2 gamma is a member of the CAMKII family.
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ADP-Glo Kinase Assay + PKC iota Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PKC iota was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C iota is a member of the protein kinase C family of serine-threonine kinases.
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ADP-Glo Kinase Assay + PDGFR beta Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human PDGFR beta (amino acids 557-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PDGFR beta is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity.
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ADP-Glo Kinase Assay + GSK3 beta Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human GSK3 beta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. GSK3 beta is a serine-threonine protein kinase originally identified as the kinase that phosphorylates and inhibits glycogen synthase.
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ADP-Glo Kinase Assay + CDK1/CyclinA2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human CDK1 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK1 is essential for completion of START, controlling event in the cell cycle required to initiate mitosis.
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ADP-Glo Kinase Assay + PDGFR alpha Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human PDGFR alpha (amino acids 550-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PDGFR alpha is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity.
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ADP-Glo Kinase Assay + PKC alpha Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PKC alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC alpha is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
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ADP-Glo Kinase Assay + GSK3 alpha Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human GSK3 alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. GSK3 alpha is a multifunctional protein serine kinase implicated in the control of several regulatory proteins.
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ADP-Glo Kinase Assay + PKC beta II Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PKC beta II was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC beta II is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
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ADP-Glo Kinase Assay + AMPK (A1/B1/G1) Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags. AMPK is an important energy-sensing enzyme that monitors cellular energy status.
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SAM510™ Colorimetric, Continuous Enzyme Coupled SAM Methyltransferase Assay, G-Biosciences
Supplier: G-Biosciences
Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation
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SAMfluoro™ Fluorescent, Continuous Enzyme Coupled SAM Methyltransferase Assay, G-Biosciences
Supplier: G-Biosciences
Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation
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CaCl₂ Solution
Supplier: Sino Biological
25 μl volume of 1 M CaCl₂ solution for different enzyme assays.