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916 results for Cellular Assays

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Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

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CytoTell™ Green, AAT BIOQUEST INC

CytoTell™ Green, AAT BIOQUEST INC

Supplier: AAT Bioquest

Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations.

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CytoTell™ Orange, AAT BIOQUEST INC

CytoTell™ Orange, AAT BIOQUEST INC

Supplier: AAT Bioquest

Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations.

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Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry with 405 nm excitation*, AAT Bioquest

Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry with 405 nm excitation*, AAT Bioquest

Supplier: AAT Bioquest

There are a variety of parameters that can be used for monitoring cell apoptosis.

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Cell Meter™ Live Cell TUNEL Apoptosis Assay Kit *Red Fluorescence* (50 Tests), AAT Bioquest

Cell Meter™ Live Cell TUNEL Apoptosis Assay Kit *Red Fluorescence* (50 Tests), AAT Bioquest

Supplier: AAT Bioquest

DNA fragmentation represents a characteristic of late stage apoptosis.

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Live or Dead™ Fixable Dead Cell Staining Kit *Deep Red Fluorescence*, AAT Bioquest

Live or Dead™ Fixable Dead Cell Staining Kit *Deep Red Fluorescence*, AAT Bioquest

Supplier: AAT Bioquest

Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions.

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GSH/GSSG-Glo Assay, 10 mL, Promega

GSH/GSSG-Glo Assay, 10 mL, Promega

Supplier: Promega Corporation

The GSH/GSSG-Glo Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells.

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ThiolTrace™ Violet 500, AAT Bioquest

ThiolTrace™ Violet 500, AAT Bioquest

Supplier: AAT Bioquest

The subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification.

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CellTiter-Glo One Solution Assay, Promega

CellTiter-Glo One Solution Assay, Promega

Supplier: Promega Corporation

The CellTiter-Glo One Solution Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, which indicates the presence of metabolically active cells.

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Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit, AAT Bioquest

Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit, AAT Bioquest

Supplier: AAT Bioquest

Lipid peroxidation is characterized by the oxidative degradation of unsaturated fatty acids, phospholipids, glycolipids, cholesterol esters and cholesterol.

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Cell Meter™ BX650 Fixable Viability Dye, AAT Bioquest

Cell Meter™ BX650 Fixable Viability Dye, AAT Bioquest

Supplier: AAT Bioquest

Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

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Nano-Glo Dual-Luciferase Reporter Assay/pGL4.53[luc2/PGK] Bundle, 1 each, Promega

Nano-Glo Dual-Luciferase Reporter Assay/pGL4.53[luc2/PGK] Bundle, 1 each, Promega

Supplier: Promega Corporation

The Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System is a homogeneous reagent system that allows you to sequentially detect the activities of firefly (Photinus pyralis) luciferase and NanoLuc luciferase (Nluc) from a single sample.

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MitoLite™ Green FM, AAT Bioquest

MitoLite™ Green FM, AAT Bioquest

Supplier: AAT Bioquest

MitoLite™ Green FM is the same molecule to the MitoTracker Green FM (M7514, ThermoFisher).

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Live-or-Dye™ Fixable Viability Staining Kits, Biotium

Live-or-Dye™ Fixable Viability Staining Kits, Biotium

Supplier: Biotium

Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry or microscopy.

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Apoptosis and Necrosis Quantitation Kits, Biotium

Apoptosis and Necrosis Quantitation Kits, Biotium

Supplier: Biotium

Biotium's apoptosis/necrosis quantitation kits pair green fluorescent FITC-Annexin V conjugate with the membrane impermeant red fluorescent nucleic acid stain ethidium homodimer III

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MycoLight™ Green JJ99 *5 mM in DMSO*, AAT Bioquest

MycoLight™ Green JJ99 *5 mM in DMSO*, AAT Bioquest

Supplier: AAT Bioquest

MycoLight™ Green JJ99 stain is an excellent green-fluorescent nuclear and chromosome counterstain that is permeant to both prokaryotic and eukaryotic cell membranes.

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ADCC Reporter Bioassay, F Variant, Core Kit, 1 each, Promega

ADCC Reporter Bioassay, F Variant, Core Kit, 1 each, Promega

Supplier: Promega Corporation

The ADCC Reporter Bioassay provides a biologically relevant and specific MOA-based measure of ADCC without the complex workflow and variability inherent in primary cell-based assays.

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Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry*, AAT Bioquest

Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry*, AAT Bioquest

Supplier: AAT Bioquest

Cell cycles have four sequential phases: G0/G1, S, G2, and M.

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Helix Fluor™ 545, Succinimidyl Ester, AAT Bioquest

Helix Fluor™ 545, Succinimidyl Ester, AAT Bioquest

Supplier: AAT Bioquest

FAM, TET, VIC, JOE, NED, TAMRA and ROX fluorescent dyes are frequently used for labeling oligos.

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Peroxidase Block, Diagnostic Biosystems

Supplier: Diagnostic Biosystems

Endogenous peroxidase activity has been observed in tissue sections during immunoperoxidase staining. If not properly suppressed, endogenous peroxidase can produce false signal as well as high background during the immunostaining. Treatment of tissue sections with Peroxidase Block prior to the application of primary antibody will suppress endogenous peroxidase activity.

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Cell Navigator® Cell Plasma Membrane Staining Kit *Red Fluorescence*, AAT Bioquest

Cell Navigator® Cell Plasma Membrane Staining Kit *Red Fluorescence*, AAT Bioquest

Supplier: AAT Bioquest

The Cell Navigator® Plasma Membrane Stain Kit provides a fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins.

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LysoBrite™ Red DND-99, AAT Bioquest

LysoBrite™ Red DND-99, AAT Bioquest

Supplier: AAT Bioquest

LysoBrite Red DND-99 is chemically same to the LysoTracker® Red DND-99 used for labeling and tracking acidic organelles in live cells (LysoTracker® is the trademark of ThermoFisher).

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CellPaint™ TSP membrane stain, AAT Bioquest

CellPaint™ TSP membrane stain, AAT Bioquest

Supplier: AAT Bioquest

TSP is a styrylpyridine-based fluorescent membrane probe suitable for imaging plasma membranes in living cells and tissues.

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Forte Blue™ DHPE, AAT Bioquest

Forte Blue™ DHPE, AAT Bioquest

Supplier: AAT Bioquest

Forte Blue™ DHPE is the same molecule to Marina Blue® 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (Marina Blue® DHPE, Marina Blue® is the trademark of ThermoFisher).

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Cell Meter™ Fluorescence Gap Junction Tracing Kit, AAT Bioquest

Cell Meter™ Fluorescence Gap Junction Tracing Kit, AAT Bioquest

Supplier: AAT Bioquest

Gap junctions are specialized intercellular membrane channels constituted with connexin that selectively facilitate the passage of small molecules of <1.5 Kd across cells.

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CF® Dye Dextran 150000 MW, Anionic and Fixable, Biotium

CF® Dye Dextran 150000 MW, Anionic and Fixable, Biotium

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 150,000 MW, and contains a formaldehyde-fixable free-amine group.

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Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Blue Fluorescence*, AAT Bioquest

Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Blue Fluorescence*, AAT Bioquest

Supplier: AAT Bioquest

The endoplasmic reticulum (ER) is a type of organelle in the cells of eukaryotic organisms that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae.

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Cell Explorer™ Live Cell Tracking Kit *Orange Fluorescence*, AAT Bioquest

Cell Explorer™ Live Cell Tracking Kit *Orange Fluorescence*, AAT Bioquest

Supplier: AAT Bioquest

Our Cell Explorer™ Live Cell Tracking Kit uses a proprietary orange fluorescent dye that gets enhanced fluorescence upon entering into live cells.

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Bodi Fluor™ R6G NHS Ester [equivalent to Bodipy® R6G, NHS Ester], AAT Bioquest

Bodi Fluor™ R6G NHS Ester [equivalent to Bodipy® R6G, NHS Ester], AAT Bioquest

Supplier: AAT Bioquest

Bodi Fluor™R6G, NHS ester is the same molecule to Bodipy® R6G, NHS ester (Bodipy® is the trademark of Invitrogen).

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Live or Dead™ Cell Viability Assay Kit *Red/Blue Dual Fluorescence*, AAT Bioquest

Live or Dead™ Cell Viability Assay Kit *Red/Blue Dual Fluorescence*, AAT Bioquest

Supplier: AAT Bioquest

This Live or Dead™ Cell Viability Assay Kit uses two fluorescent indicators: Cellbrite™ Red (Ex/Em = 613/631 nm) for labeling viable cells and a cell-impermeable DNA-binding dye Nuclear Blue™ DCS1 (Ex/Em = 360/450 nm) for labeling dead cells with damaged membranes.

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Tempo-iHepStellate™ Human iPSC-derived Hepatic Stellate Cells

Supplier: TEMPO BIOSCIENCE, INC

Hepatic stellate cells are responsible for the regulation of extracellular matrix proteins and vascular structure in the liver. They represent approximately 5 to 8% of the cells in the liver and function to maintain homeostasis in the microenvironment of the hepatic sinusoid. They play a critical role in the development of liver fibrosis. When chronic liver damage occurs, hepatic stellate cells are actively synthesizing extracellular matrix proteins such as collagen and other glycoproteins. The accumulation of the extracellular matrix proteins trigger hepatic fibrosis which can lead to cirrhosis.

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