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Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

Bucculite™ FdU Cu-Free Cell Proliferation Fluorescence Imaging Kits, AAT Bioquest

Bucculite™ FdU Cu-Free Cell Proliferation Fluorescence Imaging Kits, AAT Bioquest

Supplier: AAT Bioquest

Monitoring cell proliferation is one of the most reliable methods to assess cell viability, cell cycles and genotoxicity.

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Nano-Glo Dual-Luciferase Reporter Assay/pGL4.54[luc2/TK] Bundle, 1 each, Promega

Nano-Glo Dual-Luciferase Reporter Assay/pGL4.54[luc2/TK] Bundle, 1 each, Promega

Supplier: Promega Corporation

The Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System is a homogeneous reagent system that allows you to sequentially detect the activities of firefly (Photinus pyralis) luciferase and NanoLuc luciferase (Nluc) from a single sample.

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Caliber Fluor™ 580, AAT Bioquest

Caliber Fluor™ 580, AAT Bioquest

Supplier: AAT Bioquest

Caliber Fluor™ 580 is an extremely stable fluorophore that has its absorption and fluorescence independent of pH change from pH 3 to 10.

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PhosphoWorks™ Fluorimetric Phosphate Assay Kit, Red Fluorescence, AAT Bioquest

PhosphoWorks™ Fluorimetric Phosphate Assay Kit, Red Fluorescence, AAT Bioquest

Supplier: AAT Bioquest

Cells utilize a wide variety of phosphate (Pi) and polyphosphate esters as enzyme substrates, second messengers, membrane structural components and vital energy reservoirs.

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MitoLite™ Red CMXRos, AAT Bioquest

MitoLite™ Red CMXRos, AAT Bioquest

Supplier: AAT Bioquest

MitoLite™ Red CMXRos is chemically same to the MitoTracker™ Red CMXRos (ThermoFisher).

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Cell Meter™ BX520 Fixable Viability Dye, AAT Bioquest

Cell Meter™ BX520 Fixable Viability Dye, AAT Bioquest

Supplier: AAT Bioquest

Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

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CytoTell™ Violet 500, AAT BIOQUEST INC

CytoTell™ Violet 500, AAT BIOQUEST INC

Supplier: AAT Bioquest

Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations.

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CytoWatch™ QZ100 Monocyte Blocking Reagent, AAT Bioquest

CytoWatch™ QZ100 Monocyte Blocking Reagent, AAT Bioquest

Supplier: AAT Bioquest

Some dye-labeled fluorescent antibody conjugates used in cell surface staining of live cells often exhibit non-specific binding in monocytes and macrophages.

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Annexin V, XFD594 Conjugate, AAT Bioquest

Annexin V, XFD594 Conjugate, AAT Bioquest

Supplier: AAT Bioquest

XFD594 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher).

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CytoTrace™ Red CFDA, AAT Bioquest

CytoTrace™ Red CFDA, AAT Bioquest

Supplier: AAT Bioquest

CytoTrace™ Red fluorescent probe retains the spectral properties of Cy3/TRITC, thus its signal can be easily monitored using fluorescence microscopy or flow cytometry.

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Texas Red® Maleimide, Single Isomer, AAT Bioquest

Texas Red® Maleimide, Single Isomer, AAT Bioquest

Supplier: AAT Bioquest

Texas Red® maleimide is an excellent thiol-reactive fluorescent reagent for labeling thiol-containing biological molecules (such as peptides, proteins and thiol-modified oligonucleotides).

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Spexyte™ Micro pH Probe *Optimized for Nanodrop*, AAT Bioquest

Spexyte™ Micro pH Probe *Optimized for Nanodrop*, AAT Bioquest

Supplier: AAT Bioquest

Biological samples are usually in a very narrow pH range from 4.5 to 8.5, and many of them need to be provided in micro-volume (microliter).

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CYTO-13, AAT Bioquest

CYTO-13, AAT Bioquest

Supplier: AAT Bioquest

CYTO-13 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells.

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CytoTrace™ Ultra Green, AAT Bioquest

CytoTrace™ Ultra Green, AAT Bioquest

Supplier: AAT Bioquest

CMFDA (5-chloromethylfluorescein diacetate) has been used as a prominent cell tracker in the research community for a long time.

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Nuclear Green™ LCS1, AAT Bioquest

Nuclear Green™ LCS1, AAT Bioquest

Supplier: AAT Bioquest

Our Nuclear Green™ LCS1 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells.

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Nuclear Violet™ LCS1, AAT Bioquest

Nuclear Violet™ LCS1, AAT Bioquest

Supplier: AAT Bioquest

Our Nuclear Violet™ LCS1 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells.

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Nuclear Red™ LCS2, AAT Bioquest

Nuclear Red™ LCS2, AAT Bioquest

Supplier: AAT Bioquest

Our Nuclear Red™ LCS2 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells.

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Texas Red® Cadaverine, Single Isomer, AAT Bioquest

Texas Red® Cadaverine, Single Isomer, AAT Bioquest

Supplier: AAT Bioquest

Texas Red® cadaverine reacts with carbonyl compounds such as aldehyde, ketones and acids to give bright red fluorescent conjugates.

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ADCC Reporter Bioassay, Target (WIL2-S), 1 each, Promega

ADCC Reporter Bioassay, Target (WIL2-S), 1 each, Promega

Supplier: Promega Corporation

The ADCC Reporter Bioassay provides a biologically relevant and specific MOA-based measure of ADCC V158 without the complex workflow and variability inherent in primary cell-based assays.

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MitoLite™ Blue FX490, AAT Bioquest

MitoLite™ Blue FX490, AAT Bioquest

Supplier: AAT Bioquest

MitoLite™ Blue is a cationic dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient.

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ADCC Bioassay Effector Cells, F Variant, Propagation Model, Promega

ADCC Bioassay Effector Cells, F Variant, Propagation Model, Promega

Supplier: Promega Corporation

The ADCC Bioassay F Variant Effector Cells, Propagation Model provides a biologically relevant and specific MOA-based measure of ADCC without the complex workflow and variability inherent in primary cell-based assays.

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PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit, Blue Fluorescence, AAT Bioquest

PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit, Blue Fluorescence, AAT Bioquest

Supplier: AAT Bioquest

Pyrophosphate (PPi) is produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters.

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PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit, Enhanced Selectivity, AAT Bioquest

PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit, Enhanced Selectivity, AAT Bioquest

Supplier: AAT Bioquest

Pyrophosphate (PPi) is produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters.

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CloneDetect, Molecular Devices

Supplier: Molecular Devices

Screen and select high value mammalian clones with label-free, in situ detection of IgG secretion with the ClonePix system, and other cell analyzers with a green fluorescent channel.

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Nuclear Green™ DCS1, AAT Bioquest

Nuclear Green™ DCS1, AAT Bioquest

Supplier: AAT Bioquest

Our Nuclear Green™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analyzing DNA content in dead, fixed or apoptotic cells.

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C1-Bodi Fluor 500/510-C12 [equivalent to BODIPY® 500/510 C1, C12], AAT Bioquest

C1-Bodi Fluor 500/510-C12 [equivalent to BODIPY® 500/510 C1, C12], AAT Bioquest

Supplier: AAT Bioquest

The green fluorescent BODIPY FL C12 fatty acid can be used as a synthetic precursor to a wide variety of fluorescent phospholipids.

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pHAb Thiol Reactive Dye, Promega

pHAb Thiol Reactive Dye, Promega

Supplier: Promega Corporation

pHAb Amine Reactive Dye exhibits very low fluorescence at pH > 7 and much higher fluorescence as the pH of the solution becomes acidic. pHAb Dyes are designed specifically for antibody labeling.

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CF® Dye Dextran 250000 MW, Anionic and Fixable, Biotium

CF® Dye Dextran 250000 MW, Anionic and Fixable, Biotium

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group.

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CF® Dye Dextran 3500 MW, Anionic and Fixable, Biotium

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 3,500 MW, and contains a formaldehyde-fixable free-amine group.

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CF® Dye Dextran 40000 MW, Anionic and Fixable, Biotium

CF® Dye Dextran 40000 MW, Anionic and Fixable, Biotium

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 40,000 MW, and contains a formaldehyde-fixable free-amine group.

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