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619 results for Protein Linking and Labeling Systems

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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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SNAP-SURFACE® Alexa Fluor 488 , New England Biolabs

SNAP-SURFACE® Alexa Fluor 488 , New England Biolabs

Supplier: New England Biolabs (NEB)

SNAP-Surface® Alexa Fluor® 488 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells

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Buccutite™ Rapid APC-Cy7 Tandem Antibody Labeling Kits (2 Labelings), AAT Bioquest

Buccutite™ Rapid APC-Cy7 Tandem Antibody Labeling Kits (2 Labelings), AAT Bioquest

Supplier: AAT Bioquest

APC-Cy7 is a popular color used in flow cytometry.

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SNAP-CELL® TMR-STAR , New England Biolabs

SNAP-CELL® TMR-STAR , New England Biolabs

Supplier: New England Biolabs (NEB)

SNAP-Cell® TMR-Star is a red fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro

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Buccutite™ Rapid APC Antibody Labeling Kits (2 Labelings), AAT Bioquest

Buccutite™ Rapid APC Antibody Labeling Kits (2 Labelings), AAT Bioquest

Supplier: AAT Bioquest

APC is an red fluorescent protein which has an excitation wavelength of 651 nm and an emission wavelength of 662 nm.

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ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* (2 Labelings), AAT Bioquest

ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* (2 Labelings), AAT Bioquest

Supplier: AAT Bioquest

FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates.

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HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences

HOOK™ Amine Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences

Supplier: G-Biosciences

The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research

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HOOK™ HRP and AP Sulfo Labeling Kits for Enzyme Labeling, G-Biosciences

HOOK™ HRP and AP Sulfo Labeling Kits for Enzyme Labeling, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' HOOK™ HRP SULFO and HRP PLUS Labeling Kits are high efficiency enzyme labeling kits for tagging proteins with the horseradish peroxidase enzyme

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ReadiLink™ Rapid XFD647 Antibody Labeling Kit *XFD647 Same Structure to Alexa Fluor™ 647* (2×50 µg Labelings)

ReadiLink™ Rapid XFD647 Antibody Labeling Kit *XFD647 Same Structure to Alexa Fluor™ 647* (2×50 µg Labelings)

Supplier: AAT Bioquest

XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher).

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Buccutite™ Rapid PerCP Antibody Labeling Kits (2 Labelings), AAT Bioquest

Buccutite™ Rapid PerCP Antibody Labeling Kits (2 Labelings), AAT Bioquest

Supplier: AAT Bioquest

PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp.

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femtoCHROMO™- HRP Kit for Chromogenic Detection, G-Biosciences

femtoCHROMO™- HRP Kit for Chromogenic Detection, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' femtoCHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining

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Transcend tRNA, 30 µl, Promega

Transcend tRNA, 30 µl, Promega

Supplier: Promega Corporation

Non-Radioactive Translation Detection Systems for colorimetric or chemiluminescent detection of proteins synthesized in vitro. Biotinylated proteins are detected using Streptavidin-Alkaline Phosphatase or Streptavidin-HRP.

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Buccutite™ MTA, NHS ester (2 µmoles), AAT Bioquest

Buccutite™ MTA, NHS ester (2 µmoles), AAT Bioquest

Supplier: AAT Bioquest

Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield.

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iFluor® 350-Wheat Germ Agglutinin (WGA) Conjugate

iFluor® 350-Wheat Germ Agglutinin (WGA) Conjugate

Supplier: AAT Bioquest

iFluor® 350 WGA conjugate, like Alexa Fluor® 350 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.

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ReadiLink™ KLH Conjugation Kit (2 Reactions), AAT Bioquest

ReadiLink™ KLH Conjugation Kit (2 Reactions), AAT Bioquest

Supplier: AAT Bioquest

Keyhole Limpet Hemocyanin (KLH) is one of the most commonly used carriers in the conjugation of peptides for antibody production.

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HOOK™ Activated Agarose (Amine Reactive) for Affinity Column Generation, G-Biosciences

HOOK™ Activated Agarose (Amine Reactive) for Affinity Column Generation, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups

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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin, Biotium

Coelenterazine Substrates for Renilla Luciferase and Apoaequorin, Biotium

Supplier: Biotium

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

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ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* (2 Labelings), AAT Bioquest

ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* (2 Labelings), AAT Bioquest

Supplier: AAT Bioquest

Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed.

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ExoBrite™ STORM CTB EV Staining Kits, Biotium

ExoBrite™ STORM CTB EV Staining Kits, Biotium

Supplier: Biotium

Fluorescent cholera toxin subunit B (CTB) conjugates that are optimized for direct stochastic optical reconstruction microscopy (dSTORM) imaging of EVs and exosomes.

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Tyramide Amplification Kit with HRP Goat, Anti-Rabbit and CF® Dye or Biotin Tyramide, Biotium

Supplier: Biotium

Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.

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Pierce™ Dye Removal Columns, Thermo Scientific

Pierce™ Dye Removal Columns, Thermo Scientific

Supplier: Invitrogen

Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.

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GloCell™ Fixable Viability Dye Violet 540

GloCell™ Fixable Viability Dye Violet 540

Supplier: Stemcell Technologies

Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

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Pierce™ Sulfhydryl Addition Kit, Thermo Scientific

Pierce™ Sulfhydryl Addition Kit, Thermo Scientific

Supplier: Invitrogen

Introduce free sulfhydryl groups into a protein of interest to allow the modified protein to react with a variety of sulfhydryl-selective products.

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StabilZyme® Protein-Free AP Stabilizer, Surmodics

Supplier: Surmodics

StabilZyme® AP and StabilZyme® Protein-Free AP stabilizers provide assay developers a complete set of tools to stabilize different AP conjugates for long term, in-solution stability.

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Amine Reactive Biotinylation Kits, EZ-Link™, Thermo Scientific

Amine Reactive Biotinylation Kits, EZ-Link™, Thermo Scientific

Supplier: Invitrogen

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

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Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers, Thermo Scientific

Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers, Thermo Scientific

Supplier: Invitrogen

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

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StabilZyme® SELECT Stabilizer, Surmodics

Supplier: Surmodics

The StabilZyme® SELECT Stabilizer is a multi-purpose stabilizing solution designed to maintain the conformation of antibody/antigen conjugates, antibody coated particles and unmodified proteins that are commonly used in immunoassays. Adding the conjugate/protein directly into the SELECT Stabilizer allows for stable storage of the conjugate at a working concentration and eliminates the need for subsequent dilutions.

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AnaTag™ Biotin Microscale Protein Labeling Kit, Anaspec Inc.

Supplier: Anaspec

Biotin, SE is amino-reactive biotinylating reagent for peptides and proteins; it has a better avidin-binding affinity than biotin. Biotin, SE (d-Biotin-N-hydroxysuccinimide ester, Biotin, NHS ester) contains a five-carbon spacer arm that reduces steric hindrance associated with binding four biotinylated molecules per one avidin and results in enhanced detection sensitivity.

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Pierce™ PEGylated BM (bismaleimido) Crosslinkers, Thermo Scientific

Pierce™ PEGylated BM (bismaleimido) Crosslinkers, Thermo Scientific

Supplier: Invitrogen

Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions

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Pierce™ FITC Antibody Labeling Kit, Thermo Scientific

Pierce™ FITC Antibody Labeling Kit, Thermo Scientific

Supplier: Invitrogen

Pierce™ FITC Antibody Labeling Kit is a multi-component kit containing a high performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

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AnaTag™ HiLyte™ Fluor 488 Protein Labeling Kit [3 labeling reactions (3×5 mg protein)], Anaspec

Supplier: Anaspec

HiLyte™ Fluor 488, SE is an excellent amine-reactive fluorescent labeling dye for generating protein conjugates. The spectrum of HiLyte™ Fluor488 is similar to fluorescein (FITC), resulting in an optimal match to filters designed for fluorescein.

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