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248 results for Vectors, Plasmids and Libraries

You searched for: Vectors, Plasmids and Libraries

Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

Zika Virus Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

Zika Virus Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

Supplier: ReVacc Scientific

Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of ZIKV (GenBank: KU321639.1, SPH2015 strain) and a Renilla luciferase reporter.

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VSV-Pseudovirus_SARS-CoV-2 Omicron BF.7 Strain Spike with Luciferase Reporter

VSV-Pseudovirus_SARS-CoV-2 Omicron BF.7 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BF.7. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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pGL4.30[luc2P/NFAT-RE/Hygro] Vector, 20 µg, Promega

pGL4.30[luc2P/NFAT-RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

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pF9A CMV hRluc-neo Flexi Vector, 20 µg, Promega

pF9A CMV hRluc-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.

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pF5K CMV-neo Flexi Vector, 20 µg, Promega

pF5K CMV-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.

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pTNT Vector, 20 µg, Promega

pTNT Vector, 20 µg, Promega

Supplier: Promega Corporation

The pCMVTNT and pTNT Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from SP6- or T7-based coupled in vitro transcription/translation systems.

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pSP72 Vector, 20 µg, Promega

pSP72 Vector, 20 µg, Promega

Supplier: Promega Corporation

Used as standard cloning vector or for transcription of RNA in vitro.

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pGloSensor-20F cAMP Plasmid, 20 µg, Promega

pGloSensor-20F cAMP Plasmid, 20 µg, Promega

Supplier: Promega Corporation

The GloSensor cAMP Assay is based on a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. This live-cell assay excels at kinetic and modulation studies of signaling through cAMP.

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VSV-Pseudovirus_Lassa Virus Josiah GPC Luciferase

VSV-Pseudovirus_Lassa Virus Josiah GPC Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry glycoprotein complex (GPC) of Lassa virus (LASV) Josiah Strain (GenBank: P08669).

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pHTC HaloTag CMV-neo Vector, 20 µg, Promega

pHTC HaloTag CMV-neo Vector, 20 µg, Promega

Supplier: Promega Corporation

Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.

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pCLIPf Vector, New England Biolabs

pCLIPf Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pCLIPf Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of CLIP-tag® protein fusions in mammalian cells

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pSNAP-TAG® (T7)-2 Vector, New England Biolabs

pSNAP-TAG® (T7)-2 Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag protein

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M13mp18 RF I DNA, New England Biolabs

M13mp18 RF I DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13.

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pUC19 Vector, New England Biolabs

pUC19 Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pUC19 is a commonly used plasmid cloning vector in E.coli

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pNLCoI4[luc2-P2A-NlucP/PGK/Hygro] Vector, 20 µg, Promega

pNLCoI4[luc2-P2A-NlucP/PGK/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.

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Lenti-Pseudovirus_SARS-CoV-2 with Luciferase, ReVacc Scientific

Lenti-Pseudovirus_SARS-CoV-2 with Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses Lentiviral vectors to package full length of S protein of SARS-CoV-2 (GenBank: MN908947).

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pGL4.45[luc2P/ISRE/Hygro] Vector, 20 µg, Promega

pGL4.45[luc2P/ISRE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.

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Vsv-Pseudovirus Sars-Cov-2 Omicron Luciferase, ReVacc Scientific

Vsv-Pseudovirus Sars-Cov-2 Omicron Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron (B.1.1.529), and 18-aa cytoplasmic tail truncation for optimal infection.

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pFC30A His6HaloTag T7 Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.

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pNL2.3[secNluc/Hygro] Vector, 20 µg, Promega

pNL2.3[secNluc/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.

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pNL3.2[NlucP/minP] Vector, 20 µg, Promega

pNL3.2[NlucP/minP] Vector, 20 µg, Promega

Supplier: Promega Corporation

NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL3.1[Nluc/minP] and pNL3.2[NlucP/minP] Vectors offer a minimal promoter for cloning response elements of interest.

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pGL4.43[luc2P/XRE/Hygro] Vector, 20 µg, Promega

pGL4.43[luc2P/XRE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.

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pGL4.41[luc2P/HSE/Hygro] Vector, 20 µg, Promega

pGL4.41[luc2P/HSE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.

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pTWIN1 Vector DNA, New England Biolabs

pTWIN1 Vector DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

pTWIN1 is an E.coli expression vector which can be used with the IMPACT™ Kit (NEB #E6901).

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PhiX174 RF II DNA, New England Biolabs

PhiX174 RF II DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

This is the double-stranded, nicked, circular form of ΦX174 DNA (relaxed form)

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pGL4.32[luc2P/NF-kappa-B-RE/Hygro] Vector, 20 µg, Promega

pGL4.32[luc2P/NF-kappa-B-RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

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pmirGLO Dual-Luciferase miRNA Target Expression Vector, 20 µg, Promega

pmirGLO Dual-Luciferase miRNA Target Expression Vector, 20 µg, Promega

Supplier: Promega Corporation

The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3' of the firefly luciferase gene (luc2).

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pNL2.1[Nluc/Hygro] Vector, 20 µg, Promega

pNL2.1[Nluc/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. pNL2.1[Nluc/Hygro] and pNL2.2[NlucP/Hygro] Vectors are used for cloning putative promoters; select for stable cell lines using hygromycin.

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pGL4.33[luc2P/SRE/Hygro] Vector, 20 µg, Promega

pGL4.33[luc2P/SRE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

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pGL4.13[luc2/SV40] Vector, 20 µg, Promega

pGL4.13[luc2/SV40] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.13[luc2/SV40] ,pGL4.53[luc2/PGK] and pGL4.54[luc2/TK] Vectors are used as controls in dual-luciferase assays where Renilla or NanoLuc luciferase is the experimental reporter. Select the appropriate promoter or test all 3 in your experimental system.

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