You searched for: Vectors, Plasmids and Libraries
In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
Zika Virus Reporter Virus Particle (RVP) with Renilla Luciferase Reporter
Supplier: ReVacc Scientific
Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of ZIKV (GenBank: KU321639.1, SPH2015 strain) and a Renilla luciferase reporter.
Expand 2 Items
VSV-Pseudovirus_SARS-CoV-2 Omicron BF.7 Strain Spike with Luciferase Reporter
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BF.7. The S has 18-aa cytoplasmic tail truncation for optimal infection.
Expand 2 Items
pGL4.30[luc2P/NFAT-RE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.
Expand 1 Items
pF9A CMV hRluc-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.
Expand 1 Items
pF5K CMV-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.
Expand 1 Items
pTNT Vector, 20 µg, Promega
Supplier: Promega Corporation
The pCMVTNT and pTNT Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from SP6- or T7-based coupled in vitro transcription/translation systems.
Expand 1 Items
pSP72 Vector, 20 µg, Promega
Supplier: Promega Corporation
Used as standard cloning vector or for transcription of RNA in vitro.
Expand 1 Items
pGloSensor-20F cAMP Plasmid, 20 µg, Promega
Supplier: Promega Corporation
The GloSensor cAMP Assay is based on a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. This live-cell assay excels at kinetic and modulation studies of signaling through cAMP.
Expand 1 Items
VSV-Pseudovirus_Lassa Virus Josiah GPC Luciferase
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry glycoprotein complex (GPC) of Lassa virus (LASV) Josiah Strain (GenBank: P08669).
Expand 2 Items
pHTC HaloTag CMV-neo Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
Expand 1 Items
pCLIPf Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
pCLIPf Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of CLIP-tag® protein fusions in mammalian cells
Expand 1 Items
pSNAP-TAG® (T7)-2 Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag protein
Expand 1 Items
M13mp18 RF I DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13.
Expand 1 Items
pUC19 Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
pUC19 is a commonly used plasmid cloning vector in E.coli
Expand 2 Items
pNLCoI4[luc2-P2A-NlucP/PGK/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.
Expand 1 Items
Lenti-Pseudovirus_SARS-CoV-2 with Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses Lentiviral vectors to package full length of S protein of SARS-CoV-2 (GenBank: MN908947).
Expand 2 Items
pGL4.45[luc2P/ISRE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.
Expand 1 Items
Vsv-Pseudovirus Sars-Cov-2 Omicron Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron (B.1.1.529), and 18-aa cytoplasmic tail truncation for optimal infection.
Expand 2 Items
pFC30A His6HaloTag T7 Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.
Expand 1 Items
pNL2.3[secNluc/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.
Expand 1 Items
pNL3.2[NlucP/minP] Vector, 20 µg, Promega
Supplier: Promega Corporation
NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL3.1[Nluc/minP] and pNL3.2[NlucP/minP] Vectors offer a minimal promoter for cloning response elements of interest.
Expand 1 Items
pGL4.43[luc2P/XRE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.
Expand 1 Items
pGL4.41[luc2P/HSE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.
Expand 1 Items
pTWIN1 Vector DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
pTWIN1 is an E.coli expression vector which can be used with the IMPACT™ Kit (NEB #E6901).
Expand 1 Items
PhiX174 RF II DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
This is the double-stranded, nicked, circular form of ΦX174 DNA (relaxed form)
Expand 1 Items
pGL4.32[luc2P/NF-kappa-B-RE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.
Expand 1 Items
pmirGLO Dual-Luciferase miRNA Target Expression Vector, 20 µg, Promega
Supplier: Promega Corporation
The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3' of the firefly luciferase gene (luc2).
Expand 1 Items
pNL2.1[Nluc/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. pNL2.1[Nluc/Hygro] and pNL2.2[NlucP/Hygro] Vectors are used for cloning putative promoters; select for stable cell lines using hygromycin.
Expand 1 Items
pGL4.33[luc2P/SRE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.
Expand 1 Items
pGL4.13[luc2/SV40] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.13[luc2/SV40] ,pGL4.53[luc2/PGK] and pGL4.54[luc2/TK] Vectors are used as controls in dual-luciferase assays where Renilla or NanoLuc luciferase is the experimental reporter. Select the appropriate promoter or test all 3 in your experimental system.