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248 results for Vectors, Plasmids and Libraries

You searched for: Vectors, Plasmids and Libraries

Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

VSV-Pseudovirus_SARS-COV-2 Omicron CH.1.1 Luciferase

VSV-Pseudovirus_SARS-COV-2 Omicron CH.1.1 Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron CH.1.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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pGEM® T Vector Systems, Promega

pGEM® T Vector Systems, Promega

Supplier: Promega Corporation

The pGEM-T Vector Systems are convenient systems for cloning PCR products.

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pNLF1-NRF2 [CMV/neo] Vector, 1 each, Promega

pNLF1-NRF2 [CMV/neo] Vector, 1 each, Promega

Supplier: Promega Corporation

NanoLuc Stability Sensors are ready-to-use vector systems that utilize the advantages of the NanoLuc luciferase reporter for stability studies of two key signaling proteins, HIF1A and NRF2, providing a way to directly measure the primary signaling event.

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CheckMate Negative Control Vectors, 1 set, Promega

CheckMate Negative Control Vectors, 1 set, Promega

Supplier: Promega Corporation

Powerful system for detecting protein:protein interactions in vivo.

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pGL4.47[luc2P/SIE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.

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pGL4.12[luc2CP] Vector, 20 µg, Promega

pGL4.12[luc2CP] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.10[luc2], pGL4.11[luc2P] and pGL4.12[luc2CP] Vectors are promoterless reporter vectors optimized for expression in mammalian cells. The different luc2 genes code for luciferase proteins with different stabilities.

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pGL4.24[luc2P/minP] Vector, 20 µg, Promega

pGL4.24[luc2P/minP] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.23[luc2/minP], pGL4.24[luc2P/minP] and pGL4.25[luc2CP/minP] Vectors offer different firefly luciferase protein stabilities for expression in mammalian cells. Clone a response element of interest upstream of the minimal promoter and reporter gene.

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pNL3.1[Nluc/minP] Vector, 20 µg, Promega

pNL3.1[Nluc/minP] Vector, 20 µg, Promega

Supplier: Promega Corporation

NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL3.1[Nluc/minP] and pNL3.2[NlucP/minP] Vectors offer a minimal promoter for cloning response elements of interest.

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pLIVE® In Vivo Expression and Reporter Vector Kits, Mirus Bio

Supplier: Mirus Bio

pLIVE® vector is designed for high level, prolonged expression of transgenes in the mouse liver.

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pTYB21 Vector, New England Biolabs

pTYB21 Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pTYB21 is an E

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PhiX174 Virion DNA, New England Biolabs

PhiX174 Virion DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70

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Unmethylated Lambda DNA, New England Biolabs

Unmethylated Lambda DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7)

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pGL4.34[luc2P/SRF-RE/Hygro] Vector, 20 µg, Promega

pGL4.34[luc2P/SRF-RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

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pGL4.51[luc2/CMV/Neo] Vector, 20 µg, Promega

pGL4.51[luc2/CMV/Neo] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4 Luciferase Reporter Vectors are the next generation of luciferase reporter vectors optimized for expression in mammalian cells.

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CheckMate/Flexi Vector Mammalian Two-Hybrid System, 1 each, Promega

CheckMate/Flexi Vector Mammalian Two-Hybrid System, 1 each, Promega

Supplier: Promega Corporation

The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.

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VSV-Pseudovirus_SARS-CoV-2 Omicron BQ.1.1 Strain Spike with Luciferase Reporter

VSV-Pseudovirus_SARS-CoV-2 Omicron BQ.1.1 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BQ.1.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_SARS-CoV-2 Omicron XBB.1.5 Strain Spike with Luciferase Reporter

VSV-Pseudovirus_SARS-CoV-2 Omicron XBB.1.5 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron XBB.1.5. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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pNLCoI1[luc2-P2A-NlucP/Hygro] Vector, 20 µg, Promega

pNLCoI1[luc2-P2A-NlucP/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.

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pFC32K Nluc CMV-neo Flexi Vector, 20 µg, Promega

pFC32K Nluc CMV-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The pFN31 and pFC32 Vectors generate N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. Use pFN31A/K Nluc CMV-Hygro Flexi Vectors for N-terminal fusions and pFC32A/K Nluc CMV-Hygro Flexi Vectors for C-terminal fusions.

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pFC32A Nluc CMV-Hygro Flexi Vector, 20 µg, Promega

pFC32A Nluc CMV-Hygro Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The pFN31 and pFC32 Vectors generate N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. Use pFN31A/K Nluc CMV-Hygro Flexi Vectors for N-terminal fusions and pFC32A/K Nluc CMV-Hygro Flexi Vectors for C-terminal fusions.

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pNL1.1.PGK[Nluc/PGK] Vector, 20 µg, Promega

pNL1.1.PGK[Nluc/PGK] Vector, 20 µg, Promega

Supplier: Promega Corporation

The promoter-driven NanoLuc (Nluc) control vectors can be used to co-transfect with experimental firefly luciferase vectors when using the Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System.

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pNLF1-N [CMV/Hygro] Vector, 20 µg, Promega

pNLF1-N [CMV/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The three pNLF1 Vectors create N- or C-terminal fusions of NanoLuc luciferase and your protein of interest using a multiple cloning site (MCS). Insert your protein on the N or C terminus of NanoLuc luciferase or the N terminus of secreted NanoLuc enzyme.

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pNLF1-C [CMV/Hygro] Vector, 20 µg, Promega

pNLF1-C [CMV/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The three pNLF1 Vectors create N- or C-terminal fusions of NanoLuc luciferase and your protein of interest using a multiple cloning site (MCS). Insert your protein on the N or C terminus of NanoLuc luciferase or the N terminus of secreted NanoLuc enzyme.

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pGEM®-T Easy Vector Systems, Promega®

pGEM®-T Easy Vector Systems, Promega®

Supplier: Promega Corporation

The pGEM-T Easy Vector Systems are convenient systems for cloning PCR products. They offer all of the advantages of the pGEM-T Vector Systems with EcoRI and NotI sites flanking the insertion site.

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pGEM-3Z Vector, 20 µg, Promega

pGEM-3Z Vector, 20 µg, Promega

Supplier: Promega Corporation

Intended for use as a standard cloning vector or for high-fidelity efficient synthesis of RNA in vitro.

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CheckMate Positive Control Vectors, 1 set, Promega

CheckMate Positive Control Vectors, 1 set, Promega

Supplier: Promega Corporation

Powerful system for detecting protein:protein interactions in vivo.

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pCMVTNT Vector, 20 µg, Promega

pCMVTNT Vector, 20 µg, Promega

Supplier: Promega Corporation

The pCMVTNT and pTNT Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from SP6- or T7-based coupled in vitro transcription/translation systems.

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pFC27K HaloTag CMV-neo Flexi Vector, 20 µg, Promega

pFC27K HaloTag CMV-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Vectors designed for expression of C-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.

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pGL4.27[luc2P/minP/Hygro] Vector, 20 µg, Promega

pGL4.27[luc2P/minP/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.26[luc2/minP/Hygro], pGL4.27[luc2P/minP/Hygro] and pGL4.28[luc2CP/minP/Hygro] Vectors are used to clone a response element of interest upstream of the minimal promoter and firefly luciferase gene with and without destabilization sequences.

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pCI-neo Mammalian Expression Vector, 20 µg, Promega

pCI-neo Mammalian Expression Vector, 20 µg, Promega

Supplier: Promega Corporation

Contains a neomycin resistance gene and the CMV immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. Transfected cells can be selected with G-418.

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