You searched for: Vectors, Plasmids and Libraries
In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
pFN29A His6HaloTag T7 Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.
Expand 1 Items
pGEM-3Zf(-) Vector, 20 µg, Promega
Supplier: Promega Corporation
Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA.
Expand 1 Items
pFN11A (BIND) Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.
Expand 1 Items
pRL-Null Vector, 20 µg, Promega
Supplier: Promega Corporation
The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.
Expand 1 Items
pF1A T7 Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
These untagged vectors are used for inducible expression of proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Supplied as vectors used with the Flexi Cloning System.
Expand 2 Items
VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.75 Strain Spike with Luciferase Reporter
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA.2.75. The S has 18-aa cytoplasmic tail truncation for optimal infection.
Expand 2 Items
pGL4.26[luc2/minP/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.26[luc2/minP/Hygro], pGL4.27[luc2P/minP/Hygro] and pGL4.28[luc2CP/minP/Hygro] Vectors are used to clone a response element of interest upstream of the minimal promoter and firefly luciferase gene with and without destabilization sequences.
Expand 1 Items
pGL4.10[luc2] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.10[luc2], pGL4.11[luc2P] and pGL4.12[luc2CP] Vectors are promoterless reporter vectors optimized for expression in mammalian cells. The different luc2 genes code for luciferase proteins with different stabilities.
Expand 1 Items
VSV-Pseudovirus_SARS-CoV-2 Delta Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of B.1.617.2 (Delta strain), and 18-aa cytoplasmic tail truncation for optimal infection.
Expand 2 Items
pGL4.18[luc2P/Neo] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.17[luc2/Neo] Vector, pGL4.18[luc2P/Neo] and pGL4.19[luc2CP/Neo] Vectors are optimized for expression in mammalian cells. These vectors offer standard and destablized luciferase with neomycin for stable selection.
Expand 1 Items
VSV-Pseudovirus_SARS-CoV-2 with Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) (Wuhan strain or Wild type strain), with 18-aa cytoplasmic tail truncation for optimal infection.
Expand 2 Items
VSV-Pseudovirus_Marburg Musoke Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Musoke (GenBank: DQ217792.1).
Expand 2 Items
pNLF1-HIF1A [CMV/neo] Vector, 1 each, Promega
Supplier: Promega Corporation
NanoLuc Stability Sensors are ready-to-use vector systems that utilize the advantages of the NanoLuc luciferase reporter for stability studies of two key signaling proteins, HIF1A and NRF2, providing a way to directly measure the primary signaling event.
Expand 1 Items
pFN31A Nluc CMV-Hygro Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
The pFN31 and pFC32 Vectors generate N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. Use pFN31A/K Nluc CMV-Hygro Flexi Vectors for N-terminal fusions and pFC32A/K Nluc CMV-Hygro Flexi Vectors for C-terminal fusions.
Expand 1 Items
M13mp18 Single-Stranded DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
The single-stranded viral DNA is isolated from M13mp18
Expand 1 Items
pSNAPf Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
pSNAPf Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of SNAP-tag® protein fusions in mammalian cells
Expand 1 Items
pGL4.22[luc2CP/Puro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.20[luc2/Puro], pGL4.21[luc2P/Puro] and pGL4.22[luc2CP/Puro] Vectors are optimized for expression in mammalian cells. The luc2 genes code for luciferase proteins with different stabilities, and each vector offers puromycin for stable selection.
Expand 1 Items
pFN2K (GST) Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
These vectors are used for inducible expression of N-terminal GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.
Expand 1 Items
pGL4.54[luc2/TK] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.13[luc2/SV40] ,pGL4.53[luc2/PGK] and pGL4.54[luc2/TK] Vectors are used as controls in dual-luciferase assays where Renilla or NanoLuc luciferase is the experimental reporter. Select the appropriate promoter or test all 3 in your experimental system.
Expand 1 Items
pNLF1-secN [CMV/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The three pNLF1 Vectors create N- or C-terminal fusions of NanoLuc luciferase and your protein of interest using a multiple cloning site (MCS). Insert your protein on the N or C terminus of NanoLuc luciferase or the N terminus of secreted NanoLuc enzyme.
Expand 1 Items
psiCHECK-2 Vector, 20 µg, Promega
Supplier: Promega Corporation
psiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference assays.
Expand 1 Items
pGL4.14[luc2/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.14[luc2/Hygro], pGL4.15[luc2P/Hygro] and pGL4.16[luc2CP/Hygro] Vectors have different luc2 genes that code for luciferase proteins with different stabilities. Each vector offers hygromycin for stable selection.
Expand 1 Items
pFN10A (ACT) Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.
Expand 1 Items
pFC27A HaloTag CMV-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of C-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
Expand 1 Items
pNLCoI2[luc2-P2A-NlucP/minP/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.
Expand 1 Items
NanoBRET Positive Control, 2 × 20 µg, Promega
Supplier: Promega Corporation
The NanoBRET Positive Control Vector encodes a NanoLuc-HaloTag fusion protein that tethers together the NanoLuc donor and HaloTag acceptor proteins to ensure efficient energy transfer.
Expand 1 Items
VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.12.1 Strain Spike with Luciferase Reporter
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA.2.12.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.
Expand 2 Items
PhiX174 RF I DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
This is the double-stranded, covalently closed, circular form of ΦX174 RF I DNA (supercoiled).
Expand 2 Items
pRL-SV40 Vector, 20 µg, Promega
Supplier: Promega Corporation
The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.