You searched for: Microscopy and staining reagents

Supplier: Mopec

Amplify is a fixative spray usually applied after the application of Harmony dyes, although it can also be applied to tissue prior to marking with dyes.

Supplier: Leica Biosystems

Sub-X® Clearing Agent is an economical xylene substitute that is an essentially odorless and non-greasy aliphatic hydrocarbon.

Supplier: Chemplex

Converts powdered samples into a slurry to eliminate segregation by size or density during comminution process.

Supplier: Electron Microscopy Sciences

Cargille PCB-Free Immersion oils were introduced in 1972. Today, no other immersion oil meets FDA criteria.  Now four standard types are available with the introduction of Type NVH, a very high viscosity oil. All Cargille Immersion Oils meet DIN 58 884 (Deutsche Institut Fur Normung e.V.).

Supplier: Electron Microscopy Sciences

A water soluble embedding media which is based on Glycol Methacrylate (GMA) plastic embedding. It is intended for use in preparing samples for high resolution microscopes (HRLM). The catalyzed monomer acts as a dehydration and infiltration agent. Therefore, complete dehydration through 100% ethanol is not necessary although recommended for large or dense tissues. Conventional paraffin sections have a greater degree of shrinkage and produce inadequate morphology when compared to JB-4.JB-4 Kit Consists of 800 mL Solution A, 30 mL Solution B, and 12 g Catalyst.

Supplier: Diagnostic Biosystems

ImmunoHisto-Sealer™ is a preservation solution that acts as a sealant to improve tissue morphology and stain over an extended period of time. When applied to the stained section, specimens can be permanently mounted without chromogens fading. Additional benefits of the ImmunoHisto-Sealer™ include: saves hands on time when mounting slides, avoids the use of toxic chemicals such as xylene and eliminates accumulation of hazardous waste. This product is designed for tissue specimens stained with peroxidase, alkaline phosphatase-based systems and various chromogens. After treating with ImmunoHisto-Sealer, water solution chromogens can be mounted in Permanent Mounting Media

Supplier: Electron Microscopy Sciences

Three different mixtures: one for relatively soft blocks, another for harder blocks for collagenous tissue, and a tougher one if required., Lockwood, W.R. Anatomical Record, 150, 129 (1964). Kit consists of 2x225 ml DER 332, 225 ml DER 732, 450 ml DDSA; Specially Distilled, 50 ml DMP-30.

Supplier: AAT Bioquest

When exposed to excitation light, fluorescence intensity of dyes decreases due to their photooxidation or other photoreactions.

Supplier: AAT Bioquest

When exposed to excitation light, fluorescence intensity of dyes decreases due to their photooxidation or other photoreactions.

Supplier: Electron Microscopy Sciences

A dry temporary adhesive film, 0.003" thick, which can be easily cut to size with either a razor or scissor. This mount permits pre-placement of the adhesive film exactly where the user wants it. It melts at 195-210°F. After application it can be dissolved rapidly in trichloroethylene or toluene. This material is particularly useful in applications where a pre-formed adhesive film is needed to assure uniform bonding and flatness or in bonding stacks of substrates such as LCD glass slides for slicing. Sheet size 8"x10".

Supplier: Electron Microscopy Sciences

The same advantages offered by EPON 812, are offered by EMbed 812: rapid penetration, greater contrast, easy sectioning, stability under the electron beam, satisfactory staining of most thick sections for light microscopy and thin sections for electron microscopy.

Supplier: Electron Microscopy Sciences

Application: Mounting of Immunohisto slides

Supplier: Electron Microscopy Sciences

Hollande's fixative is a modification of Bouin's solution. Used as a fixative for biopsy specimens of the gastrointestinal tract.

Supplier: Electron Microscopy Sciences

Hatmann’s (Davidson’s) fixative is rapid fixative. Results with good nuclear detail and minimal formalin pigment.

Supplier: Electron Microscopy Sciences

CytoSeal comes in a Controlled Drop Dispenser, with varying viscosities

Supplier: Electron Microscopy Sciences

The mixture of Butyl and Methyl Methacrylate offers many advantages over the standard GMA and HPMA embedding mediums

Supplier: Electron Microscopy Sciences

UV Mount Medium is a mounting medium that is designed to match the refractive index of JB-4 embedded sections

Supplier: Electron Microscopy Sciences

In-Situ mounting medium is a unique mounting medium designed for permanent preservation of tissue sections and cell smear with alkaline phosphatase chromogens like NBT/BCIP and red stain. It is ideal for mounting tissues and cell smears.

Supplier: Electron Microscopy Sciences

Hydromount™ is the traditional choice whenever a nonfluorescing aqueous medium is needed. Hydromount™ is water-based and is suitable for mounting specimens that have been processed in water. Hydromount™ is effective for frozen sections, amyloid, and immunofluorescent staining procedures. Should it become necessary, Hydromount™ may be removed by soaking the slides in warm saline.

Supplier: Electron Microscopy Sciences

Epo-Tek® H22 is a two component, silver-filled epoxy system.

Supplier: Electron Microscopy Sciences

Technovit® 4000 is the low shrinkage embedding medium

Supplier: Electron Microscopy Sciences

The HistoTainer™ (Empty Vials, see page 482) is half filled with 10% Neutral Buffered Formalin as a fixative. These ready-to-use prefilled containers are timer saving and make your work more efficient.

Supplier: Electron Microscopy Sciences

JB-4 Plus offers all of the same features as JB-4 except it produces less of an exothermic reaction than JB4 which is good for temperature sensitive tissues

Supplier: Avantik

Can be used on all types of specimens

Supplier: Electron Microscopy Sciences

Chemkit is arranged to enable a laboratory to perform standard preparations of biological specimens.

Supplier: Electron Microscopy Sciences

Hard-Plus Resin-812 is supplied only as a premix kit

Supplier: Electron Microscopy Sciences

The kit consists of two components, equal amount, very low viscosity, (~65 cps), in convenient dispenser top bottles. To prepare the embedding resin, just mix equal amounts of the two components. The resin readily infiltrates into specimen and polymerizes to a clear, hard block overnight at 60°C.

Supplier: Mopec

Mopec’s Formaway Formalin Neutralizing Absorbent Pad (F-NAP) was designed to reduce formaldehyde vapors while dissecting tissue, transferring tissue from containers of formalin, and transporting or storing tissue in containers of formalin, by absorbing and neutralizing formalin.

Supplier: Mopec

Applied after a specimen is marked with a dye, Melody is a quick drying fixative spray that forms a protective layer on the marked surface while still allowing the tissue processing reagents to penetrate the tissue. Melody is unique to Mopec and can be used with all dye colors and on all specimen types. The chemistry of pigments, especially those seen in yellow, orange and red tissue marking dyes manufactured by alternative suppliers often suffer during tissue processing protocols.

Supplier: Electron Microscopy Sciences

DPX Mountant is a mixture of Distyrene, a plasticizer, and xylene. It is a colorless synthetic resin mounting media which replaces Xylene-Balsam. It preserves the stain and dries quickly.