You searched for: Microscopy and staining reagents

Supplier: Chemplex

Converts powdered samples into a slurry to eliminate segregation by size or density during comminution process.

Supplier: Avantik

Optik Type 2 Aqueous Clarifier is designed to be used with Type 2 Hematoxylin to produce cell transparency and remove any non-specific staining. Slightly stronger clarifier than the Optik Alcoholic Clarifier.

Supplier: Diagnostic Biosystems

Fluoromount is an innovative, aqueous-based mounting medium designed especially for the permanent preservation of fluorescent specimens.

Supplier: Electron Microscopy Sciences

Cargille PCB-Free Immersion oils were introduced in 1972. Today, no other immersion oil meets FDA criteria.  Now four standard types are available with the introduction of Type NVH, a very high viscosity oil. All Cargille Immersion Oils meet DIN 58 884 (Deutsche Institut Fur Normung e.V.).

Supplier: Diagnostic Biosystems

ImmunoHisto-Sealer™ is a preservation solution that acts as a sealant to improve tissue morphology and stain over an extended period of time. When applied to the stained section, specimens can be permanently mounted without chromogens fading. Additional benefits of the ImmunoHisto-Sealer™ include: saves hands on time when mounting slides, avoids the use of toxic chemicals such as xylene and eliminates accumulation of hazardous waste. This product is designed for tissue specimens stained with peroxidase, alkaline phosphatase-based systems and various chromogens. After treating with ImmunoHisto-Sealer, water solution chromogens can be mounted in Permanent Mounting Media

Supplier: Mopec

Amplify is a fixative spray usually applied after the application of Harmony dyes, although it can also be applied to tissue prior to marking with dyes.

Supplier: Leica Biosystems

Sub-X® Clearing Agent is an economical xylene substitute that is an essentially odorless and non-greasy aliphatic hydrocarbon.

Supplier: Electron Microscopy Sciences

Three different mixtures: one for relatively soft blocks, another for harder blocks for collagenous tissue, and a tougher one if required., Lockwood, W.R. Anatomical Record, 150, 129 (1964). Kit consists of 2x225 ml DER 332, 225 ml DER 732, 450 ml DDSA; Specially Distilled, 50 ml DMP-30.

Supplier: Electron Microscopy Sciences

EMS-Mount is a non-permanent, low viscosity an aqueous mounting medium designed for mounting of tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that cannot be dehydrated with organic solvents. This mounting medium preserves Fast red, Aminoethylycarbazole (AEC), BCIP/NBT, BCIP/INT chromogens and is also compatible with counterstain like Hematoxylin and Nuclear fast red (NFR). It is also suitable for chromogens like DAB and DAB with nickel and cobalt.

Supplier: AAT Bioquest

FluoroQuest™ PLUS Antifade mounting medium, the latest breakthrough in non-setting media technology, provides unparalleled protection against fading throughout the visible spectrum, including far-red wavelengths.

Supplier: Electron Microscopy Sciences

A dry temporary adhesive film, 0.003" thick, which can be easily cut to size with either a razor or scissor. This mount permits pre-placement of the adhesive film exactly where the user wants it. It melts at 195-210°F. After application it can be dissolved rapidly in trichloroethylene or toluene. This material is particularly useful in applications where a pre-formed adhesive film is needed to assure uniform bonding and flatness or in bonding stacks of substrates such as LCD glass slides for slicing. Sheet size 8"x10".

Supplier: Electron Microscopy Sciences

A water soluble embedding media which is based on Glycol Methacrylate (GMA) plastic embedding. It is intended for use in preparing samples for high resolution microscopes (HRLM). The catalyzed monomer acts as a dehydration and infiltration agent. Therefore, complete dehydration through 100% ethanol is not necessary although recommended for large or dense tissues. Conventional paraffin sections have a greater degree of shrinkage and produce inadequate morphology when compared to JB-4.JB-4 Kit Consists of 800 mL Solution A, 30 mL Solution B, and 12 g Catalyst.

Supplier: AAT Bioquest

When exposed to excitation light, fluorescence intensity of dyes decreases due to their photooxidation or other photoreactions.

Supplier: AAT Bioquest

When exposed to excitation light, fluorescence intensity of dyes decreases due to their photooxidation or other photoreactions.

Supplier: Electron Microscopy Sciences

EMS Shield Mount is an aqueous non-permanent, low viscosity mounting medium for immunofluorescence. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyl rhodamine and Redox. The fluorescence is retained during storage at 4 °C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes.

Supplier: Electron Microscopy Sciences

Mounting Wax 70 is a quartz type sticky, orange colored wax (translucent in thin films) used to hold crystals or other materials while they are being sliced, lapped or polished. Moderate hardness and strength make it useful for bonding small samples in diamond wheel sawing and wire sawing operations. Widely used for holding semiconductors, optical materials, ceramics and metals with medium to large contact areas. Chemical resistant. 100 Gram /Pkg of 29 sticks, .25" dia x 4" long (6mm dia x 100mm). Moderate Hardness and a Melting Point of 70°C. Dilutent: Warm soapy water

Supplier: Electron Microscopy Sciences

The same advantages offered by EPON 812, are offered by EMbed 812: rapid penetration, greater contrast, easy sectioning, stability under the electron beam, satisfactory staining of most thick sections for light microscopy and thin sections for electron microscopy.

Supplier: Electron Microscopy Sciences

Application: Mounting of Immunohisto slides

Supplier: Electron Microscopy Sciences

Hollande's fixative is a modification of Bouin's solution. Used as a fixative for biopsy specimens of the gastrointestinal tract.

Supplier: Electron Microscopy Sciences

Hatmann’s (Davidson’s) fixative is rapid fixative. Results with good nuclear detail and minimal formalin pigment.

Supplier: Electron Microscopy Sciences

CytoSeal comes in a Controlled Drop Dispenser, with varying viscosities

Supplier: Electron Microscopy Sciences

The mixture of Butyl and Methyl Methacrylate offers many advantages over the standard GMA and HPMA embedding mediums

Supplier: Electron Microscopy Sciences

UV Mount Medium is a mounting medium that is designed to match the refractive index of JB-4 embedded sections

Supplier: Electron Microscopy Sciences

In-Situ mounting medium is a unique mounting medium designed for permanent preservation of tissue sections and cell smear with alkaline phosphatase chromogens like NBT/BCIP and red stain. It is ideal for mounting tissues and cell smears.

Supplier: Electron Microscopy Sciences

Hydromount™ is the traditional choice whenever a nonfluorescing aqueous medium is needed. Hydromount™ is water-based and is suitable for mounting specimens that have been processed in water. Hydromount™ is effective for frozen sections, amyloid, and immunofluorescent staining procedures. Should it become necessary, Hydromount™ may be removed by soaking the slides in warm saline.

Supplier: Electron Microscopy Sciences

Epo-Tek® H22 is a two component, silver-filled epoxy system.

Supplier: Electron Microscopy Sciences

Technovit® 4000 is the low shrinkage embedding medium

Supplier: Electron Microscopy Sciences

The HistoTainer™ (Empty Vials, see page 482) is half filled with 10% Neutral Buffered Formalin as a fixative. These ready-to-use prefilled containers are timer saving and make your work more efficient.

Supplier: Electron Microscopy Sciences

JB-4 Plus offers all of the same features as JB-4 except it produces less of an exothermic reaction than JB4 which is good for temperature sensitive tissues

Supplier: Avantik

Can be used on all types of specimens