You searched for: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

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T4 RNA Ligase 2, New England Biolabs

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity

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Gibson Assembly Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)

Gibson Assembly was developed by Dr

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T4 RNA Ligase 1 (ssRNA Ligase) (30,000 units/ml), New England Biolabs

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 1, High Concentration (M0437M), is a 3X higher concentration than our standard T4 RNA Ligase 1 (M0204S/L)

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T4 DNA Ligase, Promega

Supplier: Promega Corporation

For ligation of DNA ends, mutagenesis and cDNA cloning

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Blunt/TA Ligase Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)

Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer.

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T4 RNA Ligase 2 Truncated, K227Q, New England Biolabs

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, truncated K227Q (T4 Rnl2tr K227Q) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

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Salt-T4 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)

A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase.

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T4 DNA Ligase, Intact Genomics

Supplier: Intact Genomics

T4 DNA ligase displays significantly higher ligation efficiency than the nearest competitor.

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Ig-Fusion Cloning Enzyme Premix, Intact Genomics

Supplier: Intact Genomics

Intact Genomics propriety Ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site.

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Taq DNA Ligase (from Thermus aquaticus cloned Escherichia coli), Intact Genomics

Supplier: Intact Genomics

Taq ligase is thermostable DNA ligase from the thermophilic bacterium Thermus aquaticus cloned to E. coli cells.

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ig-Fusion™ Cloning Kit, Intact Genomics

Supplier: Intact Genomics

Propriety ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR products to any linearized expression vector at any site.