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250 results for Vectors, Plasmids and Libraries

You searched for: Vectors, Plasmids and Libraries

Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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pFN2A (GST) Flexi Vector, 20 µg, Promega

pFN2A (GST) Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

These vectors are used for inducible expression of N-terminal GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.

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pAdVAntage Vector, 20 µg, Promega

pAdVAntage Vector, 20 µg, Promega

Supplier: Promega Corporation

Co-transfection of mammalian cells with the pAdVAntage Vector enhances transient protein expression in a variety of cell types by increasing translation initiation.

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pGL4.15[luc2P/Hygro] Vector, 20 µg, Promega

pGL4.15[luc2P/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.14[luc2/Hygro], pGL4.15[luc2P/Hygro] and pGL4.16[luc2CP/Hygro] Vectors have different luc2 genes that code for luciferase proteins with different stabilities. Each vector offers hygromycin for stable selection.

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pFN21A HaloTag CMV Flexi Vector, 20 µg, Promega

pFN21A HaloTag CMV Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.

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pF9A CMV hRluc-neo Flexi Vector, 20 µg, Promega

pF9A CMV hRluc-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.

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pGL4.30[luc2P/NFAT-RE/Hygro] Vector, 20 µg, Promega

pGL4.30[luc2P/NFAT-RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

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pNL3.3[secNluc/minP] Vector, 20 µg, Promega

pNL3.3[secNluc/minP] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.

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pGloSensor-20F cAMP Plasmid, 20 µg, Promega

pGloSensor-20F cAMP Plasmid, 20 µg, Promega

Supplier: Promega Corporation

The GloSensor cAMP Assay is based on a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. This live-cell assay excels at kinetic and modulation studies of signaling through cAMP.

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pSP72 Vector, 20 µg, Promega

pSP72 Vector, 20 µg, Promega

Supplier: Promega Corporation

Used as standard cloning vector or for transcription of RNA in vitro.

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pF5K CMV-neo Flexi Vector, 20 µg, Promega

pF5K CMV-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.

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pCLIPf Vector, New England Biolabs

pCLIPf Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pCLIPf Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of CLIP-tag® protein fusions in mammalian cells

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pSNAP-TAG® (T7)-2 Vector, New England Biolabs

pSNAP-TAG® (T7)-2 Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag protein

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pUC19 Vector, New England Biolabs

pUC19 Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pUC19 is a commonly used plasmid cloning vector in E.coli

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M13mp18 RF I DNA, New England Biolabs

M13mp18 RF I DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13.

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Zika Virus Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

Zika Virus Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

Supplier: ReVacc Scientific

Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of ZIKV (GenBank: KU321639.1, SPH2015 strain) and a Renilla luciferase reporter.

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VSV-Pseudovirus_SARS-CoV-2 Omicron BF.7 Strain Spike with Luciferase Reporter

VSV-Pseudovirus_SARS-CoV-2 Omicron BF.7 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BF.7. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_Lassa Virus Josiah GPC Luciferase

VSV-Pseudovirus_Lassa Virus Josiah GPC Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry glycoprotein complex (GPC) of Lassa virus (LASV) Josiah Strain (GenBank: P08669).

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pTNT Vector, 20 µg, Promega

pTNT Vector, 20 µg, Promega

Supplier: Promega Corporation

The pCMVTNT and pTNT Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from SP6- or T7-based coupled in vitro transcription/translation systems.

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pHTC HaloTag CMV-neo Vector, 20 µg, Promega

pHTC HaloTag CMV-neo Vector, 20 µg, Promega

Supplier: Promega Corporation

Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.

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pNL1.3.CMV[secNluc/CMV] Vector, 20 µg, Promega

pNL1.3.CMV[secNluc/CMV] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.

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pGL4.29[luc2P/CRE/Hygro] Vector, 20 µg, Promega

pGL4.29[luc2P/CRE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

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VSV-Pseudovirus_SARS-CoV-2 Alpha Luciferase, ReVacc Scientific

VSV-Pseudovirus_SARS-CoV-2 Alpha Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry spike protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations identified in variant of B.1.1.7 (Alpha strain), including: ΔH69/V70, ΔY144, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H. The spike protein has a 18-aa cytoplasmic tail truncation for optimal infection

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VSV-Pseudovirus_Marburg Angola Luciferase, ReVacc Scientific

VSV-Pseudovirus_Marburg Angola Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Angola (GenBank: KU978782.1).

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pF4K CMV Flexi Vector, 20 µg, Promega

pF4K CMV Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Designed for high-level expression of proteins in mammalian cells from the CMV promoter without a selectable marker

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pFN28A HaloTag CMV-neo Flexi Vector, 20 µg, Promega

pFN28A HaloTag CMV-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.

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pGL4.40[luc2P/MRE/Hygro] Vector, 20 µg, Promega

pGL4.40[luc2P/MRE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.

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pGL4.42[luc2P/HRE/Hygro] Vector, 20 µg, Promega

pGL4.42[luc2P/HRE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.

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pBR322 Vector, New England Biolabs

pBR322 Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pBR322 DNA is a commonly used plasmid cloning vector in E.coli.

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pTXB1 Vector DNA, New England Biolabs

pTXB1 Vector DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

pTXB1 is an E.coli expression vector in the IMPACT™ Kit.

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pGL4.74[hRluc/TK] Vector, 20 µg, Promega

pGL4.74[hRluc/TK] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.73[hRluc/SV40], pGL4.74[hRluc/TK] and pGL4.75[hRluc/CMV] Vectors are used as the control reporter for dual-luciferase assays. Select the appropriate promoter or test all three in your experimental system.

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