You searched for: Vectors, Plasmids and Libraries
In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
pGL4.27[luc2P/minP/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.26[luc2/minP/Hygro], pGL4.27[luc2P/minP/Hygro] and pGL4.28[luc2CP/minP/Hygro] Vectors are used to clone a response element of interest upstream of the minimal promoter and firefly luciferase gene with and without destabilization sequences.
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pCI-neo Mammalian Expression Vector, 20 µg, Promega
Supplier: Promega Corporation
Contains a neomycin resistance gene and the CMV immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. Transfected cells can be selected with G-418.
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pGEM-4Z Vector, 20 µg, Promega
Supplier: Promega Corporation
Intended for use as a standard cloning vector or for the high-fidelity efficient synthesis of RNA in vitro.
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pGEM-3Zf(+) Vector, 20 µg, Promega
Supplier: Promega Corporation
Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA.
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Lentivirus Pseudotyped Influenza A HPAI H5N1 virus HaNaM with Luciferase Reporter (clade 2.3.4.4b, 2022 strain)
Supplier: ReVacc Scientific
This pseudotyped virus uses lentivirus packaging to carry full length HA, NA and M of Highly Pathogenic Avian Influenza (HPAI) A virus (A/Pelecanus/Peru/VFAR-140/2022(H5N1), clade 2.3.4.4b) (GenBank: OQ565628.1). The pseudotypes can be handled using BSL-2 containment practices.
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PH.D.-7 Phage Display Peptide Library Kit v2, New England Biolabs
Supplier: New England Biolabs (NEB)
The Ph.D.−7 phage display peptide library kit v2 is based on a combinatorial library of random heptapeptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The peptide is followed by a short spacer (Gly-Gly-Gly) which directly precedes the wild-type pIII sequence. The library consists of ~1E09 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.
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Ph.D.-12 Phage Display Peptide Library Kit v2, New England Biolabs
Supplier: New England Biolabs (NEB)
The Ph.D.−12 Phage display peptide library kit v2 is based on a combinatorial library of random dodecapeptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The peptide is followed by a short spacer (Gly-Gly-Gly) which directly precedes the wild-type pIII sequence. The library consists of ~1E09 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.
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NanoBRET PPI Control Pair (p53, MDM2), 2 × 20 µg, Promega
Supplier: Promega Corporation
The NanoBRET PPI Control Pair (p53, MDM2) is an optimized pair of vectors designed to measure the interaction of protein partners p53 and MDM2 using the NanoBRET assay.
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pGL4.19[luc2CP/Neo] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.17[luc2/Neo] Vector, pGL4.18[luc2P/Neo] and pGL4.19[luc2CP/Neo] Vectors are optimized for expression in mammalian cells. These vectors offer standard and destablized luciferase with neomycin for stable selection.
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pGL4.17[luc2/Neo] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.17[luc2/Neo] Vector, pGL4.18[luc2P/Neo] and pGL4.19[luc2CP/Neo] Vectors are optimized for expression in mammalian cells. These vectors offer standard and destablized luciferase with neomycin for stable selection.
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HaloTag Control Vector, 20microg, Promega
Supplier: Promega Corporation
The HaloTag Mammalian Pull-Down Systems capture and purify intracellular protein complexes for correlative cellular localization and real-time imaging studies with the same genetic construct for further understanding of overall protein function.
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pFC14A HaloTag CMV Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of C-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
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pGL4.52[luc2P/STAT5RE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.
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pFN26A (BIND) hRluc-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
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pGL4.35[luc2P/9XGAL4UAS/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
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pNL1.1[Nluc] Vector, 20 µg, Promega
Supplier: Promega Corporation
NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL1.1[Nluc] and pNL1.2[NlucP] Vectors are used to clone putative promoter regions to express the bright NanoLuc(R) luciferase.
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pGloSensor-22F cAMP Plasmid, 20 µg, Promega
Supplier: Promega Corporation
The GloSensor cAMP Assay is based on a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. This live-cell assay excels at kinetic and modulation studies of signaling through cAMP.
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pNL2.1[Nluc/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. pNL2.1[Nluc/Hygro] and pNL2.2[NlucP/Hygro] Vectors are used for cloning putative promoters; select for stable cell lines using hygromycin.
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pGL4.33[luc2P/SRE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.
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pmirGLO Dual-Luciferase miRNA Target Expression Vector, 20 µg, Promega
Supplier: Promega Corporation
The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3' of the firefly luciferase gene (luc2).
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pGL4.13[luc2/SV40] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.13[luc2/SV40] ,pGL4.53[luc2/PGK] and pGL4.54[luc2/TK] Vectors are used as controls in dual-luciferase assays where Renilla or NanoLuc luciferase is the experimental reporter. Select the appropriate promoter or test all 3 in your experimental system.
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pNLCoI4[luc2-P2A-NlucP/PGK/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.
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pTWIN1 Vector DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
pTWIN1 is an E.coli expression vector which can be used with the IMPACT™ Kit (NEB #E6901).
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PhiX174 RF II DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
This is the double-stranded, nicked, circular form of ΦX174 DNA (relaxed form)
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pGL4.32[luc2P/NF-kappa-B-RE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.
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pGL4.70[hRluc] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4 Luciferase Reporter Vectors are the next generation of luciferase reporter vectors optimized for expression in mammalian cells.
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pFN2A (GST) Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
These vectors are used for inducible expression of N-terminal GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.
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pGL4.45[luc2P/ISRE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.
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Lenti-Pseudovirus_SARS-CoV-2 with Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses Lentiviral vectors to package full length of S protein of SARS-CoV-2 (GenBank: MN908947).
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VSV-Pseudovirus_Ebola Bomali Strain Luciferase
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry GP protein of Bombali Ebolavirus (GenBank: YP_009513277.1).
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Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: Janitorial Supplies
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: Janitorial Supplies
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International