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Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

NanoBRET Positive Control, 2 × 20 µg, Promega

NanoBRET Positive Control, 2 × 20 µg, Promega

Supplier: Promega Corporation

The NanoBRET Positive Control Vector encodes a NanoLuc-HaloTag fusion protein that tethers together the NanoLuc donor and HaloTag acceptor proteins to ensure efficient energy transfer.

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VSV-Pseudovirus_SARS-COV-2 Omicron JN.1 Luciferase

VSV-Pseudovirus_SARS-COV-2 Omicron JN.1 Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron JN.1. See mutation on our website. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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pFN2K (GST) Flexi Vector, 20 µg, Promega

pFN2K (GST) Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

These vectors are used for inducible expression of N-terminal GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.

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pFC27A HaloTag CMV-neo Flexi Vector, 20 µg, Promega

pFC27A HaloTag CMV-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Vectors designed for expression of C-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.

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psiCHECK-2 Vector, 20 µg, Promega

psiCHECK-2 Vector, 20 µg, Promega

Supplier: Promega Corporation

psiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference assays.

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pGL4.14[luc2/Hygro] Vector, 20 µg, Promega

pGL4.14[luc2/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.14[luc2/Hygro], pGL4.15[luc2P/Hygro] and pGL4.16[luc2CP/Hygro] Vectors have different luc2 genes that code for luciferase proteins with different stabilities. Each vector offers hygromycin for stable selection.

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pFN10A (ACT) Flexi Vector, 20 µg, Promega

pFN10A (ACT) Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.

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pGL4.54[luc2/TK] Vector, 20 µg, Promega

pGL4.54[luc2/TK] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.13[luc2/SV40] ,pGL4.53[luc2/PGK] and pGL4.54[luc2/TK] Vectors are used as controls in dual-luciferase assays where Renilla or NanoLuc luciferase is the experimental reporter. Select the appropriate promoter or test all 3 in your experimental system.

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pNLF1-secN [CMV/Hygro] Vector, 20 µg, Promega

pNLF1-secN [CMV/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The three pNLF1 Vectors create N- or C-terminal fusions of NanoLuc luciferase and your protein of interest using a multiple cloning site (MCS). Insert your protein on the N or C terminus of NanoLuc luciferase or the N terminus of secreted NanoLuc enzyme.

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VSV-Pseudovirus_Sudan Ebola Luciferase, ReVacc Scientific

VSV-Pseudovirus_Sudan Ebola Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry GP protein of Sudan ebolavirus strain (GenBank: KT878488.1).

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VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.12.1 Strain Spike with Luciferase Reporter

VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.12.1 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA.2.12.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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pRL-CMV Vector, 20 µg, Promega

pRL-CMV Vector, 20 µg, Promega

Supplier: Promega Corporation

The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.

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VSV-Pseudovirus_MERS-CoV Luciferase, ReVacc Scientific

VSV-Pseudovirus_MERS-CoV Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry S protein of MERS-CoV (GenBank: JX869059.2), with 16-aa cytoplasmic tail truncation for optimal infection.

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pNL3.1[Nluc/minP] Vector, 20 µg, Promega

pNL3.1[Nluc/minP] Vector, 20 µg, Promega

Supplier: Promega Corporation

NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL3.1[Nluc/minP] and pNL3.2[NlucP/minP] Vectors offer a minimal promoter for cloning response elements of interest.

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VSV-Pseudovirus_SARS-COV-2 Omicron CH.1.1 Luciferase

VSV-Pseudovirus_SARS-COV-2 Omicron CH.1.1 Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron CH.1.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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pGL4.23[luc2/minP] Vector, 20 µg, Promega

pGL4.23[luc2/minP] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.23[luc2/minP], pGL4.24[luc2P/minP] and pGL4.25[luc2CP/minP] Vectors offer different firefly luciferase protein stabilities for expression in mammalian cells. Clone a response element of interest upstream of the minimal promoter and reporter gene.

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pBIND-GR Vector, 20 µg, Promega

pBIND-GR Vector, 20 µg, Promega

Supplier: Promega Corporation

NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.

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pGL4.75[hRluc/CMV] Vector, 20 µg, Promega

pGL4.75[hRluc/CMV] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.73[hRluc/SV40], pGL4.74[hRluc/TK] and pGL4.75[hRluc/CMV] Vectors are used as the control reporter for dual-luciferase assays. Select the appropriate promoter or test all three in your experimental system.

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pFN29K His6HaloTag T7 Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.

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pGEM® T Vector Systems, Promega

pGEM® T Vector Systems, Promega

Supplier: Promega Corporation

The pGEM-T Vector Systems are convenient systems for cloning PCR products.

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CheckMate Negative Control Vectors, 1 set, Promega

CheckMate Negative Control Vectors, 1 set, Promega

Supplier: Promega Corporation

Powerful system for detecting protein:protein interactions in vivo.

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pGL4.24[luc2P/minP] Vector, 20 µg, Promega

pGL4.24[luc2P/minP] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.23[luc2/minP], pGL4.24[luc2P/minP] and pGL4.25[luc2CP/minP] Vectors offer different firefly luciferase protein stabilities for expression in mammalian cells. Clone a response element of interest upstream of the minimal promoter and reporter gene.

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pLIVE® In Vivo Expression and Reporter Vector Kits, Mirus Bio

Supplier: Mirus Bio

pLIVE® vector is designed for high level, prolonged expression of transgenes in the mouse liver.

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pGL4.12[luc2CP] Vector, 20 µg, Promega

pGL4.12[luc2CP] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.10[luc2], pGL4.11[luc2P] and pGL4.12[luc2CP] Vectors are promoterless reporter vectors optimized for expression in mammalian cells. The different luc2 genes code for luciferase proteins with different stabilities.

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pNLF1-NRF2 [CMV/neo] Vector, 1 each, Promega

pNLF1-NRF2 [CMV/neo] Vector, 1 each, Promega

Supplier: Promega Corporation

NanoLuc Stability Sensors are ready-to-use vector systems that utilize the advantages of the NanoLuc luciferase reporter for stability studies of two key signaling proteins, HIF1A and NRF2, providing a way to directly measure the primary signaling event.

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pGL4.47[luc2P/SIE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.

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PhiX174 Virion DNA, New England Biolabs

PhiX174 Virion DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70

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Unmethylated Lambda DNA, New England Biolabs

Unmethylated Lambda DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7)

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pTYB21 Vector, New England Biolabs

pTYB21 Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pTYB21 is an E

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pNL3.3[secNluc/minP] Vector, 20 µg, Promega

pNL3.3[secNluc/minP] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.

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