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250 results for Vectors, Plasmids and Libraries

You searched for: Vectors, Plasmids and Libraries

Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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pF1A T7 Flexi Vector, 20 µg, Promega

pF1A T7 Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

These untagged vectors are used for inducible expression of proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Supplied as vectors used with the Flexi Cloning System.

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pGL4.10[luc2] Vector, 20 µg, Promega

pGL4.10[luc2] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.10[luc2], pGL4.11[luc2P] and pGL4.12[luc2CP] Vectors are promoterless reporter vectors optimized for expression in mammalian cells. The different luc2 genes code for luciferase proteins with different stabilities.

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VSV-Pseudovirus_SARS-CoV-2 with Luciferase, ReVacc Scientific

VSV-Pseudovirus_SARS-CoV-2 with Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) (Wuhan strain or Wild type strain), with 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_Marburg Musoke Luciferase, ReVacc Scientific

VSV-Pseudovirus_Marburg Musoke Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Musoke (GenBank: DQ217792.1).

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pGEM-3Zf(-) Vector, 20 µg, Promega

pGEM-3Zf(-) Vector, 20 µg, Promega

Supplier: Promega Corporation

Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA.

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pFN11A (BIND) Flexi Vector, 20 µg, Promega

pFN11A (BIND) Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.

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M13mp18 Single-Stranded DNA, New England Biolabs

M13mp18 Single-Stranded DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

The single-stranded viral DNA is isolated from M13mp18

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pSNAPf Vector, New England Biolabs

pSNAPf Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pSNAPf Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of SNAP-tag® protein fusions in mammalian cells

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pF25K ICE T7 Flexi Vector, 20 µg, Promega

pF25K ICE T7 Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region to enhance translation efficiency.

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pFN29A His6HaloTag T7 Flexi Vector, 20 µg, Promega

pFN29A His6HaloTag T7 Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.

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pBR322 Vector, 10 µg, Promega

pBR322 Vector, 10 µg, Promega

Supplier: Promega Corporation

pBR322 Vector.

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pF25A ICE T7 Flexi Vector, 20 µg, Promega

pF25A ICE T7 Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region to enhance translation efficiency.

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pRL-Null Vector, 20 µg, Promega

pRL-Null Vector, 20 µg, Promega

Supplier: Promega Corporation

The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.

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pNLF1-HIF1A [CMV/neo] Vector, 1 each, Promega

pNLF1-HIF1A [CMV/neo] Vector, 1 each, Promega

Supplier: Promega Corporation

NanoLuc Stability Sensors are ready-to-use vector systems that utilize the advantages of the NanoLuc luciferase reporter for stability studies of two key signaling proteins, HIF1A and NRF2, providing a way to directly measure the primary signaling event.

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pFN31A Nluc CMV-Hygro Flexi Vector, 20 µg, Promega

pFN31A Nluc CMV-Hygro Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The pFN31 and pFC32 Vectors generate N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. Use pFN31A/K Nluc CMV-Hygro Flexi Vectors for N-terminal fusions and pFC32A/K Nluc CMV-Hygro Flexi Vectors for C-terminal fusions.

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pGL4.26[luc2/minP/Hygro] Vector, 20 µg, Promega

pGL4.26[luc2/minP/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.26[luc2/minP/Hygro], pGL4.27[luc2P/minP/Hygro] and pGL4.28[luc2CP/minP/Hygro] Vectors are used to clone a response element of interest upstream of the minimal promoter and firefly luciferase gene with and without destabilization sequences.

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pGL4.48[luc2P/SBE/Hygro] Vector, 20 µg, Promega

pGL4.48[luc2P/SBE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.

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pH6HTN His6HaloTag T7 Vector, 20 µg, Promega

pH6HTN His6HaloTag T7 Vector, 20 µg, Promega

Supplier: Promega Corporation

These multiple cloning site vectors are used for inducible expression of HaloTag-polyhistidine fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.

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pETDuet™-1 DNA Bacterial Expression Vector, EMD MilliporeSigma

Supplier: MilliporeSigma

pETDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS), each of which is preceded by a T7 promoter, lac operator and ribosome binding sites.

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VSV-Pseudovirus_SARS-COV-2 Omicron CH.1.1 Luciferase

VSV-Pseudovirus_SARS-COV-2 Omicron CH.1.1 Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron CH.1.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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pGEM® T Vector Systems, Promega

pGEM® T Vector Systems, Promega

Supplier: Promega Corporation

The pGEM-T Vector Systems are convenient systems for cloning PCR products.

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CheckMate Negative Control Vectors, 1 set, Promega

CheckMate Negative Control Vectors, 1 set, Promega

Supplier: Promega Corporation

Powerful system for detecting protein:protein interactions in vivo.

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pRL-CMV Vector, 20 µg, Promega

pRL-CMV Vector, 20 µg, Promega

Supplier: Promega Corporation

The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.

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VSV-Pseudovirus_MERS-CoV Luciferase, ReVacc Scientific

VSV-Pseudovirus_MERS-CoV Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry S protein of MERS-CoV (GenBank: JX869059.2), with 16-aa cytoplasmic tail truncation for optimal infection.

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PhiX174 Virion DNA, New England Biolabs

PhiX174 Virion DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70

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Unmethylated Lambda DNA, New England Biolabs

Unmethylated Lambda DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7)

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pTYB21 Vector, New England Biolabs

pTYB21 Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pTYB21 is an E

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pGL4.47[luc2P/SIE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.

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pNLF1-NRF2 [CMV/neo] Vector, 1 each, Promega

pNLF1-NRF2 [CMV/neo] Vector, 1 each, Promega

Supplier: Promega Corporation

NanoLuc Stability Sensors are ready-to-use vector systems that utilize the advantages of the NanoLuc luciferase reporter for stability studies of two key signaling proteins, HIF1A and NRF2, providing a way to directly measure the primary signaling event.

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pLIVE® In Vivo Expression and Reporter Vector Kits, Mirus Bio

Supplier: Mirus Bio

pLIVE® vector is designed for high level, prolonged expression of transgenes in the mouse liver.

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