Media for Microbiology

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Microbiology media facilitate the detection, isolation, and cultivation of microorganism colonies. Culture media additives and supplements enhance the growth capability of specific organisms. Prepared nutrient broths help establish the complex environment that even fastidious organisms require. Containing the nutritional elements that enable cells to multiply, the predetermined substance quantities add additional control to tests. Choose the gel or liquid microbiology media that is most compatible with working cell types.

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Phenol Red Sucrose Broth

Description: A basal medium containing a single carbohydrate used to determine whether bacteria can ferment the specific carbohydrate. The medium contains a pH indicator that changes from red to yellow if the bacteria can ferment the carbohydrate.

Phenol Red Dextrose Broth

Description: To prepare media, add 20 g of phenol red dextrose broth to a liter of distilled or de-ionized water. The media will not be sterile until autoclaved prior to use.

Phenol Red Lactose Broth

Starch Agar

Description: Used to determine if bacteria produce extracellular enzymes to catalyze the hydrolysis of starch. Organisms that produce a starch hydrolyzing enzyme will display a clear zone around the area of growth while other areas of the agar containing starch will turn blue-black.

Simmons Citrate Agar

Description: A medium helpful in differentiation of the enterobacteriaceae. Simmons citrate agar test for organisms that are able to utilize citrate as a carbon source.

Sim Medium

Description: To assist in the differentiation of enteric organisms the medium provides three tests - indole production, production of hydrogen sulfide by organisms that can reduce sulfur, and motility.