Biotechnology-based diagnostic tests are beginning to supplement existing medical tests. In this experiment, students determine a pedigree for a family suspected to be carriers of mutations in their p53 genes. This is followed by a diagnostic agarose gel analysis to diagnose the state of the p53 gene in individual family members. Students gain an understanding of the p53 tumor suppressor gene and its role in familial cancers.
Includes materials for six student groups to run six gels of five lanes each. Requires, but does not include horizontal gel electrophoresis apparatus, DC power supply, and automatic micropipets with tips.