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Plant Pigments and Photosynthesis Lab Activity |
| Includes Easy-to-Use Chromatography Paper Strips and Vials |
With a thorough overview of photosynthesis, chromatography, and spectrophotometry, this comprehensive lab activity helps students perform all the required investigations on plant pigments and photosynthesis. In the process, students learn the principles of chromatography, calculate Rƒ values, and carry out experiments that show how light intensity, light wavelength, and temperature affect the rate of photosynthesis. After learning the proper procedure for extracting chloroplasts from spinach leaves, students separate plant pigments with paper chromatography and study the pigments’ relation to photosynthesis. In addition, they measure the rate of photosynthesis in isolated chloroplasts using a unique method that involves the reduction of the dye DPIP (2,6-dichloroindophenol). The change in color from blue to clear, effected by the transfer of electrons, is measured with a spectrophotometer, available separately.
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