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WARD’S Sordaria Genetics Lab Activity |
| Classic Experiment to Demonstrate Crossing Over |
Students will be able to identify genetic differences between diploid and haploid organisms, explain how genetic variability arises from sexual reproduction, and study the roles of mitosis and meiosis. Students will grow wild type and mutant Sordaria strains on mating agar and examine the ascospores under a microscope (available separately). They will then calculate the distance in map units between the gene for ascospore color and the chromosome centromere. The kit includes a teacher’s guide, student copymaster, and enough materials for eight setups. Note: Coupon included for perishable materials. Redeem by mail, fax, phone, or e-mail.
Materials Included
•2 Bottles of Sordaria mating agar
•10 Petri plates
•72 Microscope slides
•100 Coverslips
•16 Sterile scalpels
•15 Disposable pipets
•8 Disposable inoculating loops
•1 Redemption coupon for: 1 Sordaria fimicola plate, wild type, 1 Sordaria fimicola plate, tan mutant
Materials Needed But Not Provided
•Compound microscope
•30ºC incubator
Time Requirement
•Part I: 30 minutes
•Part II: 45 minutes
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