Restriction enzymes, one of the primary tools in molecular biology, cut DNA molecules into precise fragments of a predictable size. Students can see the results by digesting lambda DNA with restriction enzymes PvuII and EcoR I and running a gel, then determining the basepair size of each fragment. The activity can be completed in two 60 minute sessions. The kit includes enough materials to run eight gels, a teacher’s guide, and student copymaster. An electrophoresis chamber, power supply, 10 μL micropipets, and UV transilluminator (for ethidium bromide kit only) required, are available separately. Note: Coupon included for perishable materials. Redeem by mail, fax, phone, or e-mail. Ethidium bromide version sold only to colleges, universities, and accredited laboratories. Check your local regulations for disposal of ethidium bromide.
Materials Included
•1 Bottle of 0.8% agarose, 200 mL
•1 Bottle of 5X TBE running buffer concentrate, 500 mL
•1 Bottle of WARD’S QUIKView DNA stain concentrate, 60 mL (36 W 8850)
•1 Tube of Ethidium Bromide stain concentrate, 50 ?L (36 W 9950)
•1 Biohazard bag (36 W 9950)
•30 Microfuge tubes
•8 Gel staining trays
•Loading dye, 0.5 mL
•1 Coupon redeemable for: 1 Lambda DNA/Hind III marker, 80 ?L, 3 Lambda DNA, 80 ?L, EcoR I restriction enzyme, 50 ?L, Pvu II restriction enzyme, 50 ?L, 10X EcoR I reaction buffer , 50 ?L, 10X Pvu II reaction buffer , 50 ?L
Materials Needed But Not Provided
•Electrophoresis chamber
•Power supply
•Incubator or hot water bath
•Micropipets
•Metric ruler
•Permanent marker
•Calculator
•gloves
•goggles
•apron
•UV Transilluminator (36 W 9950)
•UV goggles (36 W 9950)
Time Requirement
•Part I: 90 minutes
•Part II: 120 minutes
Tell a Friend about this item
|
